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Transcytosis mechanisms of cell-penetrating peptides: Cation-independent CC12 and cationic penetratin

文献类型：期刊论文

作者Zhu, Minjiao2; Liu, Haiyang3; Cao, Wenjiao2; Fang, Yuefei4; Chen, Zheng2; Qi, Xinming1; Luo, Dawei5; Chen, Chong5
刊名JOURNAL OF PEPTIDE SCIENCE
出版日期2022-02-15
页码9
ISSN号1075-2617
关键词cell-penetrating peptides direct translocation pathway drug delivery endocytic pathway penetration transcytosis cell internalization mechanism
DOI10.1002/psc.3408
通讯作者Chen, Chong(cicyheart@163.com)
英文摘要Cell-penetrating peptides (CPPs) can aid in intracellular and in vivo drug delivery. However, the mechanisms of CPP-mediated penetration remain unclear, limiting the development and further application of CPPs. Flow cytometry and laser confocal fluorescence microscopy were performed to detect the effects of different endocytosis inhibitors on the internalization of CC12 and penetratin in ARPE-19 cells. The co-localization of CPPs with the lysosome and macropinosome was detected via an endocytosis tracing experiment. The flow cytometry results showed that chlorpromazine, wortmannin, cytochalasin D, and the ATP inhibitor oligomycin had dose-dependent endocytosis-inhibitory effects on CC12. The laser confocal fluorescence results showed that oligomycin had the most significant inhibitory effect on CC12 uptake; CC12 was co-located with the lysosome, but not with the macropinosome. For penetratin, cytochalasin D and oligomycin had obvious inhibitory effects. The laser confocal fluorescence results indicated that oligomycin had the most significant inhibitory effect on penetratin uptake; the co-localization of penetratin with the lysosome was higher than that with the macropinosome. Cation-independent CC12 and cationic penetratin may be internalized into cells primarily through caveolae and clathrin-mediated endocytosis, and they are typically dependent on ATP. The transport of penetratin could be partly achieved through the direct transmembrane pathway, as the positive charge of penetratin interacts with the negative charge of the cell membrane, and partly through the endocytic pathway.
WOS关键词TAT PEPTIDE ; PROTEINS ; ENDOCYTOSIS ; DELIVERY
资助项目Shanghai Sailing Program[18YF1419700] ; National Natural Science Foundation of China[81800835] ; National Natural Science Foundation of China[81970810] ; Shanghai Jiao Tong University[YG2021QN136] ; Shanghai Jiao Tong University[YG2019QNA59] ; Shanghai Rising Stars of Medical Talent Youth Development Program ; Shanghai Talent Development Fund
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者WILEY
WOS记录号WOS:000755360000001
源URL[http://119.78.100.183/handle/2S10ELR8/300418]  
专题新药研究国家重点实验室
通讯作者Chen, Chong
作者单位1.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Key Lab Embryo Original Dis, Shanghai Municipal Key Clin Specialty, Sch Med,Dept Obstet & Gynecol,Int Peace Matern &, Shanghai, Peoples R China
3.Peoples Hosp Dongtai, Dept Resp Med, Dongtai, Jiangsu, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
5.Shanghai Jiao Tong Univ, Shanghai Gen Hosp,Shanghai Engn Ctr Visual Sci &, Sch Med,Shanghai Key Lab Ocular Fundus Dis, Dept Ophthalmol,Natl Clin Res Ctr Eye Dis,Shangha, 100 Haining Rd, Shanghai 200080, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Minjiao,Liu, Haiyang,Cao, Wenjiao,et al. Transcytosis mechanisms of cell-penetrating peptides: Cation-independent CC12 and cationic penetratin[J]. JOURNAL OF PEPTIDE SCIENCE,2022:9.
APA Zhu, Minjiao.,Liu, Haiyang.,Cao, Wenjiao.,Fang, Yuefei.,Chen, Zheng.,...&Chen, Chong.(2022).Transcytosis mechanisms of cell-penetrating peptides: Cation-independent CC12 and cationic penetratin.JOURNAL OF PEPTIDE SCIENCE,9.
MLA Zhu, Minjiao,et al."Transcytosis mechanisms of cell-penetrating peptides: Cation-independent CC12 and cationic penetratin".JOURNAL OF PEPTIDE SCIENCE (2022):9.

入库方式： OAI收割

来源：上海药物研究所

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