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Dual-Channel Fluorescent Probe for the Simultaneous Monitoring of Peroxynitrite and Adenosine-5 '-triphosphate in Cellular Applications

文献类型：期刊论文

作者Wu, Luling4,5,6,7; Liu, Jihong6; Tian, Xue7; Groleau, Robin R.7; Feng, Beidou3; Yang, Yonggang3; Sedgwick, Adam C.8; Han, Hai-Hao1,2; Wang, Yang4,5; Wang, Han-Min9
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2021-12-21
页码10
ISSN号0002-7863
DOI10.1021/jacs.1c07954
通讯作者Han, Hai-Hao(hanhaihao@126.com) ; Zhang, Hua(zhanghua1106@163.com) ; Huang, Chusen(huangcs@shnu.edu.cn) ; Li, Ping(lip@sdnu.edu.cn) ; James, Tony D.(t.d.james@bath.ac.uk) ; Sessler, Jonathan L.(sessler@cm.utexas.edu)
英文摘要Changes in adenosine triphosphate (ATP) and peroxynitrite (ONOO-) concentrations have been correlated in a number of diseases including ischemia-reperfusion injury and drug-induced liver injury. Herein, we report the development of a fluorescent probe ATP-LW, which enables the simultaneous detection of ONOO- and ATP. ONOO- selectively oxidizes the boronate pinacol ester of ATP-LW to afford the fluorescent 4-hydroxy-1,8-naphthalimide product NA-OH (lambda(ex) = 450 nm, lambda(em) = 562 nm or lambda(ex) = 488 nm, lambda(em) = 568 nm). In contrast, the binding of ATP to ATP-LW induces the spirolactam ring opening of rhodamine to afford a highly emissive product (lambda(ex) = 520 nm, lambda(em) = 587 nm). Due to the differences in emission between the ONOO- and ATP products, ATP-LW allows ONOO- levels to be monitored in the green channel (lambda(ex) = 488 nm, lambda(em) = 500-575 nm) and ATP concentrations in the red channel (lambda(ex) = 514 nm, lambda(em) = 575-650 nm). The use of ATP-LW as a combined ONOO- and ATP probe was demonstrated using hepatocytes (HL-7702 cells) in cellular imaging experiments. Treatment of HL-7702 cells with oligomycin A (an inhibitor of ATP synthase) resulted in a reduction of signal intensity in the red channel and an increase in that of the green channel as expected for a reduction in ATP concentrations. Similar fluorescence changes were seen in the presence of SIN-1 (an exogenous ONOO- donor).
WOS关键词INDUCED LIVER-INJURY ; COUMARIN-BASED FLUORESCENT ; SENSITIVE K+-CHANNELS ; REACTIVE OXYGEN ; ATP ; APOPTOSIS ; MITOCHONDRIA ; GLUTATHIONE ; MECHANISM ; BRAIN
资助项目China Scholarship Council ; University of Bath ; EPSRC Centre for Doctoral Training in Catalysis[EP/L016443/1] ; EPSRC ; Royal Society ; Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University[2020ZD01] ; National Natural Science Foundation of China[21927811] ; National Natural Science Foundation of China[22074083] ; National Natural Science Foundation of China[21672150] ; National Natural Science Foundation of China[21302125] ; National Natural Science Foundation of China[21722501] ; National Natural Science Foundation of China[11974103] ; Key Research and Development Program of Shandong Province[2018YFJH0502] ; National Science Foundation of Shandong Province of China[ZR2020ZD17] ; Alexander von Humboldt Foundation (AvH) ; Shanghai Rising-Star Program[19QA1406400] ; Shanghai Government[18DZ2254200] ; China Postdoctoral Science Foundation[2020M681196] ; National Natural Science Foundation[91853201] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; National Key Sci-Tech Special Projects of Infection Diseases of China[2018ZX10732202] ; International Cooperation Program of Shanghai Science and Technology Committee[17520750100] ; Zhongyuan High Level Talents Special Support Plan-Science and Technology Innovation Leading Talents[204200510006] ; Program for Science Technology Innovation Talents in Universities of Henan Province[21HASTIT019] ; Robert A. Welch Foundation[F-0018]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000736110100001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/300667]  
专题新药研究国家重点实验室
通讯作者Han, Hai-Hao; Zhang, Hua; Huang, Chusen; Li, Ping; James, Tony D.; Sessler, Jonathan L.
作者单位1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, Shanghai 200237, Peoples R China
2.East China Univ Sci & Technol, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai 200237, Peoples R China
3.Henan Normal Univ, Sch Phys, Xinxiang 453007, Henan, Peoples R China
4.Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Educ Minist, Key Lab Resource Chem, Shanghai 200234, Peoples R China
5.Shanghai Normal Univ, Dept Chem, Shanghai Municipal Educ Comm, Key Lab Mol Imaging Probes & Sensors, Shanghai 200234, Peoples R China
6.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Minist Educ,Inst Biomed, Jinan 250014, Peoples R China
7.Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
8.Univ Texas Austin, Dept Chem, Austin, TX 78712 USA
9.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Luling,Liu, Jihong,Tian, Xue,et al. Dual-Channel Fluorescent Probe for the Simultaneous Monitoring of Peroxynitrite and Adenosine-5 '-triphosphate in Cellular Applications[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2021:10.
APA Wu, Luling.,Liu, Jihong.,Tian, Xue.,Groleau, Robin R..,Feng, Beidou.,...&Sessler, Jonathan L..(2021).Dual-Channel Fluorescent Probe for the Simultaneous Monitoring of Peroxynitrite and Adenosine-5 '-triphosphate in Cellular Applications.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,10.
MLA Wu, Luling,et al."Dual-Channel Fluorescent Probe for the Simultaneous Monitoring of Peroxynitrite and Adenosine-5 '-triphosphate in Cellular Applications".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021):10.

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来源：上海药物研究所

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