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PDE4B Induces Epithelial-to-Mesenchymal Transition in Bladder Cancer Cells and Is Transcriptionally Suppressed by CBX7

文献类型：期刊论文

作者Huang, Zhengnan2; Liu, Jiakuan3; Yang, Jiale4,5; Yan, Yilin6; Yang, Chenkai6; He, Xiao1,4; Huang, Ruimin1,4,5; Tan, Mingyue7; Wu, Denglong2; Yan, Jun3
刊名FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
出版日期2021-12-16
卷号9页码:12
关键词phosphodiesterase 4B urinary bladder cancer chromobox protein homolog 7 epithelial-to-mesenchymal transition beta-catenin
ISSN号2296-634X
DOI10.3389/fcell.2021.783050
通讯作者Wu, Denglong(wudenglong2009@tongji.edu.cn) ; Yan, Jun(yan_jun@fudan.edu.cn) ; Shen, Bing(urodrshenbing@shsmu.edu.cn)
英文摘要Urinary bladder cancer (UBC) is a common malignant tumor with high incidence. Advances in the diagnosis and treatment of this disease demand the identification of novel therapeutic targets. Multiple studies demonstrated that PDE4B level was upregulated in malignancies and high PDE4B expression was correlated with poor outcomes. Herein, we identified that PDE4B was a potential therapeutic target in UBC. We confirmed that PDE4B expression was correlated with aggressive clinicopathological characteristics and unfavorable prognosis. Functional studies demonstrated that ectopic expression of PDE4B promoted UBC cells proliferation, migration and invasion, whereas PDE4B depletion suppressed cancer cell aggressiveness. We also identified CBX7 as a regulator of PDE4B to suppress the expression of PDE4B at the transcription level in a PRC1-dependent manner. Moreover, our results indicated that PDE4B induced epithelial-to-mesenchymal transition (EMT) in UBC cells via beta-catenin pathway, whereas inhibition of PDE4B by its small molecule inhibitor, rolipram, effectively reversed the PDE4B overexpression-induced effects. To sum up, our results indicated that PDE4B acts as an oncogene by promoting UBC cell migration and invasion via beta-catenin/EMT pathway.
WOS关键词PHOSPHODIESTERASE PDE4B ; CAMP ; PROGRESSION ; MECHANISMS ; EXPRESSION ; PLASTICITY ; PATHWAY ; CATENIN ; WNT ; EMT
资助项目National Natural Science Foundation[81972370] ; National Natural Science Foundation[82072821] ; National Natural Science Foundation[92059112] ; Shanghai Songjiang Municipal Science and Technology Commission Natural Science Foundation[20SJKJGG250]
WOS研究方向Cell Biology ; Developmental Biology
语种英语
WOS记录号WOS:000739056100001
出版者FRONTIERS MEDIA SA
源URL[http://119.78.100.183/handle/2S10ELR8/300688]  
专题中国科学院上海药物研究所
通讯作者Wu, Denglong; Yan, Jun; Shen, Bing
作者单位1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Peoples R China
2.Tongji Univ, Tongji Hosp, Dept Urol, Sch Med, Shanghai, Peoples R China
3.Fudan Univ, Dept Lab Anim Sci, Shanghai, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
5.Univ Chinese Acad Sci, Sch Pharm, Beijing, Peoples R China
6.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Urol, Sch Med, Shanghai, Peoples R China
7.Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Urol, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Zhengnan,Liu, Jiakuan,Yang, Jiale,et al. PDE4B Induces Epithelial-to-Mesenchymal Transition in Bladder Cancer Cells and Is Transcriptionally Suppressed by CBX7[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2021,9:12.
APA Huang, Zhengnan.,Liu, Jiakuan.,Yang, Jiale.,Yan, Yilin.,Yang, Chenkai.,...&Shen, Bing.(2021).PDE4B Induces Epithelial-to-Mesenchymal Transition in Bladder Cancer Cells and Is Transcriptionally Suppressed by CBX7.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,9,12.
MLA Huang, Zhengnan,et al."PDE4B Induces Epithelial-to-Mesenchymal Transition in Bladder Cancer Cells and Is Transcriptionally Suppressed by CBX7".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9(2021):12.

入库方式： OAI收割

来源：上海药物研究所

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