A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria
文献类型：期刊论文
| 作者 | Yu, Liang2,3 ; Cao, Qiao3; Chen, Weizhong4; Yang, Nana2,3; Yang, Cai-Guang1,2,3; Ji, Quanjiang4; Wu, Min5; Bae, Taeok6; Lan, Lefu1,2,3,7
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| 刊名 | SCIENCE BULLETIN
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| 出版日期 | 2022-01-30 |
| 卷号 | 67期号:2页码:198-212 |
| ISSN号 | 2095-9273 |
| 关键词 | Pseudomonas aeruginosa Two-component system Disulfide bond formation Gene regulation Copper resistance |
| DOI | 10.1016/j.scib.2021.03.003 |
| 通讯作者 | Lan, Lefu(llan@simm.ac.cn) |
| 英文摘要 | In nature, bacteria must sense copper and tightly regulate gene expression to evade copper toxicity. Here, we identify a new copper-responsive two-component system named DsbRS in the important human pathogen Pseudomonas aeruginosa; in this system, DsbS is a sensor histidine kinase, and DsbR, its cognate response regulator, directly induces the transcription of genes involved in protein disulfide bond formation (Dsb) (i.e., the dsbDEG operon and dsbB). In the absence of copper, DsbS acts as a phosphatase toward DsbR, thus blocking the transcription of Dsb genes. In the presence of copper, the metal ion directly binds to the sensor domain of DsbS, and the Cys82 residue plays a critical role in this process. The copperbinding behavior appears to inhibit the phosphatase activity of DsbS, leading to the activation of DsbR. The copper resistance of the dsbRS knock-out mutant is restored by the ectopic expression of the dsbDEG operon, which is a DsbRS major target. Strikingly, cognates of the dsbRS-dsbDEG pair are widely distributed across eubacteria. In addition, a DsbR-binding site, which contains the consensus sequence 50TTA-N8-TTAA-30, is detected in the promoter region of dsbDEG homologs in these species. These findings suggest that the regulation of Dsb genes by DsbRS represents a novel mechanism by which bacterial cells cope with copper stress. (c) 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved. |
| WOS关键词 | DISULFIDE BOND FORMATION ; PSEUDOMONAS-AERUGINOSA ; ESCHERICHIA-COLI ; RESISTANCE MECHANISMS ; SENSOR ; BINDING ; PROTEINS ; NETWORKS ; CUSS |
| 资助项目 | National Key R&D Program of China[2016YFA0501503] ; National Mega-project for Innovative Drugs of China[2019ZX09721001-004-003] ; National Natural Science Foundation of China[31670136] ; National Natural Science Foundation of China[31870127] ; National Natural Science Foundation of China[81861138047] ; Science and Technology Commission of Shanghai Municipality[19JC1416400] ; State Key Laboratory of Drug Research[SIMM2003ZZ-03] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000740434600014 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/300700]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Lan, Lefu |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 5.Univ North Dakota, Dept Biomed Sci, Grand Forks, ND 58203 USA 6.Indiana Univ, Dept Microbiol & Immunol, Sch Med Northwest, Gary, IN 46408 USA 7.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Liang,Cao, Qiao,Chen, Weizhong,et al. A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria[J]. SCIENCE BULLETIN,2022,67(2):198-212. |
| APA | Yu, Liang.,Cao, Qiao.,Chen, Weizhong.,Yang, Nana.,Yang, Cai-Guang.,...&Lan, Lefu.(2022).A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria.SCIENCE BULLETIN,67(2),198-212. |
| MLA | Yu, Liang,et al."A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria".SCIENCE BULLETIN 67.2(2022):198-212. |
入库方式： OAI收割
来源：上海药物研究所
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