中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Preclinical evaluation of [Tc-99m]Tc-labeled anti-EpCAM nanobody for EpCAM receptor expression imaging by immuno-SPECT/CT

文献类型：期刊论文

作者Liu, Tianyu2; Wu, Yue2; Shi, Linqing2; Li, Liqiang2; Hu, Biao2; Wang, Yanpu2; Gao, Hannan2; Yu, Xiaolu3; Zhang, Xin2; Zhao, Huiyun2
刊名EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
出版日期2022-01-11
页码12
关键词Immuno-SPECT/CT EpCAM imaging [Tc-99m]Tc-Nanobody Site-specific radiolabeling
ISSN号1619-7070
DOI10.1007/s00259-021-05670-z
通讯作者Jia, Bing(jiabing@bjmu.edu.cn) ; Wang, Fan(wangfan@bjmu.edu.cn)
英文摘要Purpose Overexpression of epithelial cell adhesion molecule (EpCAM) plays essential roles in tumorigenesis and tumor progression in almost all epithelium-derived cancer. Monitoring EpCAM expression in tumors can be used for the diagnosis, staging, and prognosis of cancer patients, as well as guiding the individualized treatment of EpCAM-targeted drugs. In this study, we described the synthesis and evaluation of a site-specifically [Tc-99m]Tc-labeled EpCAM-targeted nanobody for the SPECT/CT imaging of EpCAM expression. Methods We first prepared the [Tc-99m]Tc-HYNIC-G(4)K; then, it was site-specifically connected to EpCAM-targeted nanobody NB4. The in vitro characteristics of [Tc-99m]Tc-NB4 were investigated in HT-29 (EpCAM positive) and HL-60 (EpCAM negative) cells, while the in vivo studies were performed using small-animal SPECT/CT in the subcutaneous tumor models and the lymph node metastasis model to verify the specific targeting capacity as well as the potential applications of [Tc-99m]Tc-NB4. Results [Tc-99m]Tc-NB4 displayed a high EpCAM specificity both in vitro and in vivo. SPECT/CT imaging revealed that [Tc-99m]Tc-NB4 was cleared rapidly from the blood and normal organs except for the kidneys, and HT-29 tumors were clearly visualized in contrast with HL-60 tumors. The uptake value of [Tc-99m]Tc-NB4 in HT-29 tumors was increased continuously from 3.77 +/- 0.39%ID/g at 0.5 h to 5.53 +/- 0.82%ID/g at 12 h after injection. Moreover, the [Tc-99m]Tc-NB4 SPECT/CT could clearly image tumor-draining lymph nodes. Conclusion [Tc-99m]Tc-NB4 is a broad-spectrum, specific, and sensitive SPECT radiotracer for the noninvasive imaging of EpCAM expression in the epithelium-derived cancer and revealed a great potential for the clinical translation.
WOS关键词CELL ADHESION MOLECULE ; EP-CAM ; CANCER ; PROSTATE ; COLON
资助项目National Natural Science Foundation of China (NSFC)[81871416] ; National Natural Science Foundation of China (NSFC)[81630045] ; National Natural Science Foundation of China (NSFC)[81927802] ; National Natural Science Foundation of China (NSFC)[81621063] ; National Key R&D Program of China[2017YFA0205600]
WOS研究方向Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:000741570200001
出版者SPRINGER
源URL[http://119.78.100.183/handle/2S10ELR8/300721]  
专题新药研究国家重点实验室
通讯作者Jia, Bing; Wang, Fan
作者单位1.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China
2.Peking Univ, Med Isotopes Res Ctr, Sch Basic Med Sci, Dept Radiat Med, Beijing 100191, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Shanghai Novamab Biopharmaceut Co Ltd, Shanghai, Peoples R China
5.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Tianyu,Wu, Yue,Shi, Linqing,et al. Preclinical evaluation of [Tc-99m]Tc-labeled anti-EpCAM nanobody for EpCAM receptor expression imaging by immuno-SPECT/CT[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2022:12.
APA Liu, Tianyu.,Wu, Yue.,Shi, Linqing.,Li, Liqiang.,Hu, Biao.,...&Wang, Fan.(2022).Preclinical evaluation of [Tc-99m]Tc-labeled anti-EpCAM nanobody for EpCAM receptor expression imaging by immuno-SPECT/CT.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,12.
MLA Liu, Tianyu,et al."Preclinical evaluation of [Tc-99m]Tc-labeled anti-EpCAM nanobody for EpCAM receptor expression imaging by immuno-SPECT/CT".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2022):12.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。