Histone lysine methacrylation is a dynamic post-translational modification regulated by HAT1 and SIRT2
文献类型：期刊论文
| 作者 | Delaney, Kyle9; Tan, Minjia1,9 ; Zhu, Zhesi4; Gao, Jinjun9; Dai, Lunzhi2,3,9; Kim, Sunjoo5; Ding, Jun9; He, Maomao4; Halabelian, Levon6; Yang, Lu9
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| 刊名 | CELL DISCOVERY
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| 出版日期 | 2021-12-28 |
| 卷号 | 7期号:1页码:14 |
| DOI | 10.1038/s41421-021-00344-4 |
| 通讯作者 | Zhao, Yingming(yingming.zhao@uchicago.edu) |
| 英文摘要 | Histone lysine crotonylation is a posttranslational modification with demonstrated functions in transcriptional regulation. Here we report the discovery of a new type of histone posttranslational modification, lysine methacrylation (Kmea), corresponding to a structural isomer of crotonyllysine. We validate the identity of this modification using diverse chemical approaches and further confirm the occurrence of this type of histone mark by pan specific and site-specific anti-methacryllysine antibodies. In total, we identify 27 Kmea modified histone sites in HeLa cells using affinity enrichment with a pan Kmea antibody and mass spectrometry. Subsequent biochemical studies show that histone Kmea is a dynamic mark, which is controlled by HAT1 as a methacryltransferase and SIRT2 as a de-methacrylase. Altogether, these investigations uncover a new type of enzyme-catalyzed histone modification and suggest that methacrylyl-CoA generating metabolism is part of a growing number of epigenome-associated metabolic pathways. |
| WOS关键词 | METABOLIC-REGULATION ; GENE-EXPRESSION ; SUBSTRATE-SPECIFICITY ; CROTONYLATION ; ACETYLTRANSFERASE ; ACETYLATION ; CHROMATIN ; YEAST ; TRANSCRIPTION ; MALONYLATION |
| 资助项目 | University of Chicago ; NIH[R01GM135504] ; NIH[R01DK118266] ; NIH[R01GM062970] ; NIH[RO1AR078555] ; Fondation ARC[PGA1RF2019208471] ; ANR Episperm4 program ; NSF[1808087] ; Nancy and Leonard Florsheim family fund ; Plan Cancer[CH7INS15B66] ; Plan Cancer[C7H-KIC19N30-IAB] ; Plan Cancer[ASC16012CSA] ; INCa-IReSP[R19051CC] ; University Grenoble Alpes[ANR-15-IDEX-02] ; AbbVie ; Bayer Pharma AG ; Boehringer Ingelheim ; Canada Foundation for Innovation ; Eshelman Institute for Innovation ; Genentech ; Genome Canada through Ontario Genomics Institute[OGI-196] ; EU/EFPIA/OICR/McGill/KTH/Diamond Innovative Medicines Initiative 2 Joint Undertaking [EUbOPEN grant][875510] ; Janssen ; Novartis Pharma AG ; Pfizer ; Sao Paulo Research Foundation-FAPESP ; Takeda ; Wellcome Trust ; Merck Co. |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000734796300001 |
| 出版者 | SPRINGERNATURE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/300827]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhao, Yingming |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Sichuan Univ, West China Hosp, Dept Gen Practice, State Key Lab Biotherapy, Chengdu, Peoples R China 3.Collaborat Innovat Ctr Biotherapy, Chengdu, Peoples R China 4.Univ Georgia, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA 5.Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu, South Korea 6.Univ Toronto, Struct Genom Consortium, Toronto, ON, Canada 7.Ohio State Univ, Dept Biol Chem & Pharmacol, Columbus, OH 43210 USA 8.Univ Grenoble Alpes, Inst Adv Biosci, CNRS, INSERM,U1209,UMR 5309, Grenoble, France 9.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Delaney, Kyle,Tan, Minjia,Zhu, Zhesi,et al. Histone lysine methacrylation is a dynamic post-translational modification regulated by HAT1 and SIRT2[J]. CELL DISCOVERY,2021,7(1):14. |
| APA | Delaney, Kyle.,Tan, Minjia.,Zhu, Zhesi.,Gao, Jinjun.,Dai, Lunzhi.,...&Zhao, Yingming.(2021).Histone lysine methacrylation is a dynamic post-translational modification regulated by HAT1 and SIRT2.CELL DISCOVERY,7(1),14. |
| MLA | Delaney, Kyle,et al."Histone lysine methacrylation is a dynamic post-translational modification regulated by HAT1 and SIRT2".CELL DISCOVERY 7.1(2021):14. |
入库方式： OAI收割
来源：上海药物研究所
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