Chondrogenic preconditioning of mesenchymal stem/stromal cells within a magnetic scaffold for osteochondral repair
文献类型：期刊论文
| 作者 | Zhang,Jiabin1,2; Zhang,Ming4; Lin,Rongcai3; Du,Yuguang7; Wang,Liming3; Yao,Qingqiang3; Zannettino,Andrew5,8; Zhang,Hu2,6 |
| 刊名 | Biofabrication
 |
| 出版日期 | 2022-03-14 |
| 卷号 | 14期号:2 |
| 关键词 | magnetic nanofibers chondrogenesis osteogenesis mesenchymal stem/stromal cells osteochondral repair |
| ISSN号 | 1758-5082 |
| DOI | 10.1088/1758-5090/ac5935 |
| 英文摘要 | Abstract Stem cell therapy using mesenchymal stem/stromal cells (MSCs) represents a novel approach to treating severe diseases, including osteoarthritis. However, the therapeutic benefit of MSCs is highly dependent on their differentiation state, which can be regulated by many factors. Herein, three-dimensional (3D) magnetic scaffolds were successfully fabricated by incorporating magnetic nanoparticles (MNPs) into electrospun gelatin nanofibers. When positioned near a rotating magnet (f = 0.5 Hz), the magnetic scaffolds with the embedded MSCs were driven upward/downward in the culture container, which induced mechanical stimulation to MSCs due to spatial confinement and fluid flow. The extracellular matrix-mimicking scaffold and the alternating magnetic field significantly enhanced chondrogenesis instead of osteogenesis. Furthermore, the fiber topography could be tuned with different compositions of the coating layer on MNPs, and the topography had a significant impact on MSC differentiation. Selective up-regulation of chondrogenesis-related genes (COL2A1 and ACAN) was found for the magnetic scaffolds with citric acid-coated MNPs (CAG). In contrast, osteogenesis-related genes (RUNX2 and SPARC) were selectively and significantly up-regulated for the magnetic scaffolds with polyvinylpyrrolidone-coated MNPs. Prior to implantation in vivo, chondrogenic preconditioning of MSCs within the CAG scaffolds under a dynamic magnetic field resulted in superior osteochondral repair. Hence, the magnetic scaffolds together with an in-house rotating magnet device could be a novel platform to initiate multiple stimuli on stem cell differentiation for effective repair of osteochondral defects. |
| 语种 | 英语 |
| WOS记录号 | IOP:BF_14_2_025020 |
| 出版者 | IOP Publishing |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/49985]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Yao,Qingqiang; Zannettino,Andrew; Zhang,Hu |
| 作者单位 | 1.Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, People’s Republic of China 2.School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia 3.Department of Orthopaedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, People’s Republic of China 4.Department of Orthopedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu Province, People’s Republic of China 5.Adelaide Medical School, The University of Adelaide, Adelaide, SA 5001, Australia 6.Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, California, CA 91711, United States of America 7.Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China 8.Central Adelaide Local Health Network, Adelaide, SA 5000, Australia |
| 推荐引用方式 GB/T 7714 | Zhang,Jiabin,Zhang,Ming,Lin,Rongcai,et al. Chondrogenic preconditioning of mesenchymal stem/stromal cells within a magnetic scaffold for osteochondral repair[J]. Biofabrication,2022,14(2). |
| APA | Zhang,Jiabin.,Zhang,Ming.,Lin,Rongcai.,Du,Yuguang.,Wang,Liming.,...&Zhang,Hu.(2022).Chondrogenic preconditioning of mesenchymal stem/stromal cells within a magnetic scaffold for osteochondral repair.Biofabrication,14(2). |
| MLA | Zhang,Jiabin,et al."Chondrogenic preconditioning of mesenchymal stem/stromal cells within a magnetic scaffold for osteochondral repair".Biofabrication 14.2(2022). |
入库方式： OAI收割
来源：过程工程研究所
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