Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation
文献类型：期刊论文
| 作者 | Jia, Yunpu1,2; Samak, Nadia A.1,2,3,5; Hao, Xuemi1,2; Chen, Zheng1,2; Yang, Gama1,2; Zhao, Xuhao1; Mu, Tingzhen1; Yang, Maohua1; Xing, Jianmin1,2,4
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| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
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| 出版日期 | 2021-12-01 |
| 卷号 | 176页码:8 |
| ISSN号 | 1369-703X |
| 关键词 | Plastic biodegradation Immobilization of biocatalyst Biomimetic mineralization |
| DOI | 10.1016/j.bej.2021.108205 |
| 英文摘要 | PET hydmlase (PETase), discovered in Ideonella sakaiensis, is a promising agent for the biodegradation of polyethylene terephthalate (PET) capable of PET decomposition under mild reaction conditions with limited stability and productivity. Here, the immobilization of His-tagged PETase was achieved by synthesizing enzyme-inorganic nanoflowers, PETase@Co-3(PO4)(2), which was designed based on the principle of biomimetic mineralization. Immobilization of PETase onto nanostructured Co-3(PO4)(2) enjoys high enzyme loading and low mass transfer inhibition due to large specific surface area, high movement speed, and large surface curvature caused by small particle size. The nano-effect of inorganic carriers materialize the 10 degrees C optimum temperature swelling of the immobilized PETase with enhanced pH tolerance (6.0-10.0) than the free counterpart. The long-duration reaction showed that the productivity of terephthalic acid (TPA) was 3.5 times higher than that of the free enzyme. PETase@Co-3(PO4)(2) still retained 75% of the initial activity after 12 days compared with the free enzymes, which showed almost no activity. The excellent and stable catalytic performance of PETase@Co-3(PO4)( )2 with low cost demonstrates the synthetical usefulness of immobilization via biomimetic mineralization in the enzyme utilization in industrial PET depolymerization. |
| WOS关键词 | BIOMIMETIC MINERALIZATION ; NANOFLOWERS ; HYDROLASE ; CUTINASE ; BINDING |
| 资助项目 | National Natural Science Foundation of China[31961133017] ; National Natural Science Foundation of China[31961133018] ; National Natural Science Foundation of China[31961133019] ; European Union's Horizon 2020 research and innovation program[870294] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000697108600031 |
| 资助机构 | National Natural Science Foundation of China ; European Union's Horizon 2020 research and innovation program |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/50260]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Xing, Jianmin |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Coll Chem Engn, Beijing 100049, Peoples R China 3.Univ Duisburg Essen, Aquat Microbiol, Environm Microbiol & Biotechnol, D-4141 Essen, Germany 4.Chem & Chem Engn Guangdong Lab, Shantou 515031, Peoples R China 5.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt |
| 推荐引用方式 GB/T 7714 | Jia, Yunpu,Samak, Nadia A.,Hao, Xuemi,et al. Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation[J]. BIOCHEMICAL ENGINEERING JOURNAL,2021,176:8. |
| APA | Jia, Yunpu.,Samak, Nadia A..,Hao, Xuemi.,Chen, Zheng.,Yang, Gama.,...&Xing, Jianmin.(2021).Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation.BIOCHEMICAL ENGINEERING JOURNAL,176,8. |
| MLA | Jia, Yunpu,et al."Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation".BIOCHEMICAL ENGINEERING JOURNAL 176(2021):8. |
入库方式： OAI收割
来源：过程工程研究所
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