Porous Au@Pt nanoparticles with superior peroxidase-like activity for colorimetric detection of spike protein of SARS-CoV-2
文献类型：期刊论文
| 作者 | Fu, Zhao1; Zeng, Weilun1,2; Cai, Shuangfei1; Li, Haolin1,2; Ding, Jianwei1; Wang, Chen1; Chen, Yunfa3; Han, Ning3; Yang, Rong1,2 |
| 刊名 | JOURNAL OF COLLOID AND INTERFACE SCIENCE
 |
| 出版日期 | 2021-12-15 |
| 卷号 | 604页码:113-121 |
| 关键词 | Nanozyme Catalysis Mechanism Colorimetric Detection SARS-CoV-2 spike protein |
| ISSN号 | 0021-9797 |
| DOI | 10.1016/j.jcis.2021.06.170 |
| 英文摘要 | The development of colorimetric assays for rapid and accurate diagnosis of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is of practical importance for point-of-care (POC) testing. Here we report the colorimetric detection of spike (S1) protein of SARS-CoV-2 based on excellent peroxidase-like activity of Au@Pt nanoparticles, with merits of rapidness, easy operation, and high sensitivity. The Au@Pt NPs were fabricated by a facile seed mediated growth approach, in which spherical Au NPs were premade as seeds, followed by the Pt growth on Au seeds, producing uniform, monodispersed and porous Au@Pt core-shell NPs. The as-obtained Au@Pt NPs showed a remarkable enhancement in the peroxidase-mimic catalysis, which well abided by the typical Michaelis-Menten theory. The enhanced catalysis of Au@Pt NPs was ascribed to the porous nanostructure and formed electron-rich Pt shells, which enabled the catalytic pathway to switch from hydroxyl radical generation to electron transfer process. On a basis of these findings, a colorimetric assay of spike (S1) protein of SARS-CoV-2 was established, with a linear detection range of 10-100 ng mL-1 of protein concentration and a low limit of detection (LOD) of 11 ng mL-1. The work presents a novel strat-egy for diagnosis of COVID-19 based on metallic nanozyme-catalysis. (c) 2021 Elsevier Inc. All rights reserved. |
| WOS关键词 | BIMETALLIC NANOPARTICLES ; CATALYTIC-ACTIVITY ; GRAPHENE OXIDE ; NANOMATERIALS ; NANOCLUSTERS ; COVID-19 ; NANOZYME ; GLUCOSE |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB36000000] ; National key research and development program from the Ministry of Science and Technology of China[2016YFC0207102] ; National Natural Science Foundation of China[21503053] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000704428600003 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; National key research and development program from the Ministry of Science and Technology of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/50385]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Cai, Shuangfei; Yang, Rong |
| 作者单位 | 1.Univ Chinese Acad Sci, Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, Ctr Mat Sci & Optoelect Engn,CAS Key Lab Biomed E, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Sino Danish Ctr Educ & Res, Sino Danish Coll, Beijing, Peoples R China 3.Chinese Acad Sci, State Key Lab Multiphase Complex Syst, Inst Proc Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Zhao,Zeng, Weilun,Cai, Shuangfei,et al. Porous Au@Pt nanoparticles with superior peroxidase-like activity for colorimetric detection of spike protein of SARS-CoV-2[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2021,604:113-121. |
| APA | Fu, Zhao.,Zeng, Weilun.,Cai, Shuangfei.,Li, Haolin.,Ding, Jianwei.,...&Yang, Rong.(2021).Porous Au@Pt nanoparticles with superior peroxidase-like activity for colorimetric detection of spike protein of SARS-CoV-2.JOURNAL OF COLLOID AND INTERFACE SCIENCE,604,113-121. |
| MLA | Fu, Zhao,et al."Porous Au@Pt nanoparticles with superior peroxidase-like activity for colorimetric detection of spike protein of SARS-CoV-2".JOURNAL OF COLLOID AND INTERFACE SCIENCE 604(2021):113-121. |
入库方式： OAI收割
来源：过程工程研究所
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