Exceptionally rich keratinolytic enzyme profile found in the rare actinomycetes Amycolatopsis keratiniphila D2(T)
文献类型：期刊论文
| 作者 | Espersen, Roall1,2; Huang, Yuhong3,4; Falco, Francesco C.5; Hagglund, Per1,6; Gernaey, Krist, V5; Lange, Lene3,7; Svensson, Birte1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2021-10-04 |
| 页码 | 10 |
| ISSN号 | 0175-7598 |
| 关键词 | Keratinolytic enzymes PPR functional genome annotation MEROPS families Proteomics Azo-keratin assay Biological degradation process |
| DOI | 10.1007/s00253-021-11579-2 |
| 英文摘要 | The non-spore forming Gram-positive actinomycetes Amycolatopsis keratiniphila subsp. keratiniphila D2(T) (DSM 44,409) has a high potential for keratin valorization as demonstrated by a novel biotechnological microbial conversion process consisting of a bacterial growth phase and a keratinolytic phase, respectively. Compared to the most gifted keratinolytic Bacillus species, a very large number of 621 putative proteases are encoded by the genome of Amycolatopsis keratiniphila subsp. keratiniphila D2(T), as predicted by using Peptide Pattern Recognition (PPR) analysis. Proteome analysis by using LC-MS/MS on aliquots of the supernatant of A. keratiniphila subsp. keratiniphila D2(T) culture on slaughterhouse pig bristle meal, removed at 24, 48, 96 and 120 h of growth, identified 43 proteases. This was supplemented by proteome analysis of specific fractions after enrichment of the supernatant by anion exchange chromatography leading to identification of 50 proteases. Overall 57 different proteases were identified corresponding to 30% of the 186 proteins identified from the culture supernatant and distributed as 17 metalloproteases from 11 families, including an M36 protease, 38 serine proteases from 4 families, and 13 proteolytic enzymes from other families. Notably, M36 keratinolytic proteases are prominent in fungi, but seem not to have been discovered in bacteria previously. Two S01 family peptidases, named T- and C-like proteases, prominent in the culture supernatant, were purified and shown to possess a high azo-keratin/azo-casein hydrolytic activity ratio. The C-like protease revealed excellent thermostability, giving promise for successful applications in biorefinery processes. Notably, the bacterium seems not to secrete enzymes for cleavage of disulfides in the keratinous substrates. |
| WOS关键词 | CARBOHYDRATE-ACTIVE ENZYMES ; M12 METALLOPROTEASE ; SP NOV. ; KERATINASE ; BACTERIUM ; STRAIN ; DECOMPOSITION ; DEGRADATION ; HYDROLYSIS ; MYROILYSIN |
| 资助项目 | Innovation Fund Denmark | The Program Commission on Health, Food and Welfare[1308-00015B] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000703372500001 |
| 资助机构 | Innovation Fund Denmark | The Program Commission on Health, Food and Welfare |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/50418]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Svensson, Birte |
| 作者单位 | 1.Tech Univ Denmark, Dept Biotechnol & Biomed, Enzyme & Prot Chem, Soltofts Plads Bldg 224, DK-2800 Lyngby, Denmark 2.Statens Serum Inst, Ctr Vaccine Res, Artillerivej 5 Bldg 81, DK-2300 Copenhagen S, Denmark 3.Tech Univ Denmark, Dept Chem & Biochem Engn, Soltofts Plads Bldg 227, DK-2800 Lyngby, Denmark 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Multiphase Complex Syst, Beijing Key Lab Ion Liquids Clean Proc,Key Lab Gr, Beijing 100190, Peoples R China 5.Tech Univ Denmark, Proc & Syst Engn Ctr, Dept Chem & Biochem Engn, Soltofts Plads Bldg 228 A, DK-2800 Lyngby, Denmark 6.Univ Copenhagen, Dept Biomed Sci, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark 7.Bioecon Res & Advisory, Karensgade 5, DK-2500 Valby, Denmark |
| 推荐引用方式 GB/T 7714 | Espersen, Roall,Huang, Yuhong,Falco, Francesco C.,et al. Exceptionally rich keratinolytic enzyme profile found in the rare actinomycetes Amycolatopsis keratiniphila D2(T)[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2021:10. |
| APA | Espersen, Roall.,Huang, Yuhong.,Falco, Francesco C..,Hagglund, Per.,Gernaey, Krist, V.,...&Svensson, Birte.(2021).Exceptionally rich keratinolytic enzyme profile found in the rare actinomycetes Amycolatopsis keratiniphila D2(T).APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,10. |
| MLA | Espersen, Roall,et al."Exceptionally rich keratinolytic enzyme profile found in the rare actinomycetes Amycolatopsis keratiniphila D2(T)".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2021):10. |
入库方式： OAI收割
来源：过程工程研究所
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