Novel enzyme-metal-organic framework composite for efficient cadaverine production
文献类型：期刊论文
| 作者 | Yao, Jiahao1,2; Li, Zhuang1; Ji, Xiuling1; Xue, Yaju1; Ren, Baozeng2; Zhao, Hai3; Huang, Yuhong1,3,4,5 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 176页码:8 |
| 关键词 | Enzyme-MOF composite LdcEt@UIO-66-NH2 Cadaverine Lysine decarboxylase Immobilization |
| ISSN号 | 1369-703X |
| DOI | 10.1016/j.bej.2021.108422 |
| 英文摘要 | Cadaverine is an important precursor for the synthesis of nylon 5X, which is used for engineering plastics and fibers. L-lysine can be converted to cadaverine by lysine decarboxylases with high catalytic activity and selectivity. However, the pH sensitivity and non-recyclability impede the practical application of lysine decarboxylases for cadaverine production. In this study, lysine decarboxylase (LdcEt) was immobilized on the surface of the zirconium-based metal-organic framework material (MOF) UIO-66-NH2 via covalent linkage for the first time. Morphological and structural analysis of the enzyme-MOF composite (LdcEt@HIO-66-NH2) indicated that LdcEt was successfully immobilized on the surface of UIO-66-NH2. Meanwhile, LdcEt@HIO-66-NH2 showed higher catalytic efficiency and enzyme activity, broader pH and temperature reaction range than free LdcEt. Compared with free enzymes, LdcEt@HIO-66-NH2 could catalyze more cadaverine in 2 h at high-level substrate concentration (365.3 g L-1 ). Furthermore, LdcEt@HIO-66-NH2 can also be reused for 10 cycles. These results proved that the efficient enzyme-MOF composite LdcEt@HIO-66-NH2 has great potential for the large scale cadaverine production. |
| WOS关键词 | LYSINE DECARBOXYLASE ; ENHANCED ACTIVITY ; IMMOBILIZATION ; LIPASE ; MOF ; ENCAPSULATION ; STABILITY ; LACCASE |
| 资助项目 | National Natural Science Foundation of China[22078346] ; Beijing Nova Program of Science and Technology[Z201100006820141] ; Innovation Academy for Green Manufacture, CAS[IAGM2020C19] ; Henan Key Research and Development Project[202102210046] ; Hebei Natural Science Foundation[B2020103010] ; CAS Pioneer Hundred Program |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000710065000002 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; Beijing Nova Program of Science and Technology ; Innovation Academy for Green Manufacture, CAS ; Henan Key Research and Development Project ; Hebei Natural Science Foundation ; CAS Pioneer Hundred Program |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/50726]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Huang, Yuhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, CAS Key Lab Green Proc & Engn,State Key Lab Multi, Beijing 100190, Peoples R China 2.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450000, Henan, Peoples R China 3.Chinese Acad Sci, Innovat Acad Green Manufacture, Beijing 100190, Peoples R China 4.Zhengzhou Zhongke Emerging Ind Technol Res Inst, Zhengzhou 450000, Henan, Peoples R China 5.Zhongke Langfang Inst Proc Engn, Langfang 065001, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Jiahao,Li, Zhuang,Ji, Xiuling,et al. Novel enzyme-metal-organic framework composite for efficient cadaverine production[J]. BIOCHEMICAL ENGINEERING JOURNAL,2021,176:8. |
| APA | Yao, Jiahao.,Li, Zhuang.,Ji, Xiuling.,Xue, Yaju.,Ren, Baozeng.,...&Huang, Yuhong.(2021).Novel enzyme-metal-organic framework composite for efficient cadaverine production.BIOCHEMICAL ENGINEERING JOURNAL,176,8. |
| MLA | Yao, Jiahao,et al."Novel enzyme-metal-organic framework composite for efficient cadaverine production".BIOCHEMICAL ENGINEERING JOURNAL 176(2021):8. |
入库方式： OAI收割
来源：过程工程研究所
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