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Self-assembled peptide nanoparticles for enhanced dark-field hyperspectral imaging at the cellular and invertebrate level

文献类型：期刊论文

作者Liu, Yamei2; Naumenko, Ekaterina4; Akhatova, Farida4; Zou, Qianli2; Fakhrullin, Rawil4; Yan, Xuehai1,2,3
刊名CHEMICAL ENGINEERING JOURNAL
出版日期2021-11-15
卷号424页码:8
关键词Peptide Covalent self-assembly Dark-field hyperspectral imaging Cell tracking Turbatrix aceti nematodes
ISSN号1385-8947
DOI10.1016/j.cej.2021.130348
英文摘要Enhanced dark-field hyperspectral imaging (EDF-HSI) integrates advanced spectroscopy and imaging techniques, enabling fast and quantitative insights into the interactions between nanomaterials and organisms at the cellular or invertebrate level. However, the usage of biomolecule-based nanomaterials as contrast agents for EDF-HSI is highly challenging because these nanomaterials usually lack the characteristic optical scattering properties that can be readily distinguished from the scattering signals of organisms. Herein, we report peptide nanoparticles fabricated through covalent self-assembly of dipeptides and genipin for EDF-HSI in vitro and in vivo. Two dipeptides with either C-terminal amidation or not were used as the building blocks for covalent self-assembly. Significantly, the optical scattering intensity of the resulting nanoparticles is enhanced by the amidation. The amidation also enhances the stability of the peptide nanoparticles both in solutions and inside cells or organisms due to inhibition of electrostatic repulsion among the building blocks. Therefore, the nanoparticles fabricated from the dipeptide with uncharged C-terminal amide can be facilely visualized and tracked inside cells and nematodes through EDF-HSI. These results demonstrate that peptide nanomaterials can be endowed with optical scattering properties suitable for EDF-HSI, providing a promising approach for visualization and analysis of the activities of biomaterials in vitro and in vivo.
WOS关键词PLASMONIC NANOPARTICLES ; MICROSCOPY ; GRAPHENE
资助项目National Natural Science Foundation of China[22025207] ; National Natural Science Foundation of China[21977095] ; Chinese Postdoctoral Science Foundation[2020M680674] ; Russian Fund for Basic Research[18-53-80067 BRICS_t] ; National Natural Science Fund BRICS STI Framework Program[51861145304] ; Key Research Program of Frontier Sciences of Chinese Academy of Sciences (CAS)[QYZDB-SSW-JSC034]
WOS研究方向Engineering
语种英语
WOS记录号WOS:000707037200044
出版者ELSEVIER SCIENCE SA
资助机构National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Russian Fund for Basic Research ; National Natural Science Fund BRICS STI Framework Program ; Key Research Program of Frontier Sciences of Chinese Academy of Sciences (CAS)
源URL[http://ir.ipe.ac.cn/handle/122111/50788]  
专题中国科学院过程工程研究所
通讯作者Zou, Qianli; Fakhrullin, Rawil; Yan, Xuehai
作者单位1.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Ctr Mesosci, Beijing 100190, Peoples R China
4.Kazan Fed Univ, Inst Fundamental Med & Biol, Kazan 420008, Republic Of Tat, Russia
推荐引用方式
GB/T 7714		 Liu, Yamei,Naumenko, Ekaterina,Akhatova, Farida,et al. Self-assembled peptide nanoparticles for enhanced dark-field hyperspectral imaging at the cellular and invertebrate level[J]. CHEMICAL ENGINEERING JOURNAL,2021,424:8.
APA Liu, Yamei,Naumenko, Ekaterina,Akhatova, Farida,Zou, Qianli,Fakhrullin, Rawil,&Yan, Xuehai.(2021).Self-assembled peptide nanoparticles for enhanced dark-field hyperspectral imaging at the cellular and invertebrate level.CHEMICAL ENGINEERING JOURNAL,424,8.
MLA Liu, Yamei,et al."Self-assembled peptide nanoparticles for enhanced dark-field hyperspectral imaging at the cellular and invertebrate level".CHEMICAL ENGINEERING JOURNAL 424(2021):8.

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来源：过程工程研究所

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