Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice
文献类型：期刊论文
| 作者 | Ge, Enhui1; Zha, Rui1,2; Gao, Qing1,2; Guo, Lirong1; Wang, Binglong1; Lin, Qiqi1; Zhang, Guifeng3; Xie, Weiquan4,5; Yin, Hui1; Liu, Tao1 |
| 刊名 | JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
 |
| 出版日期 | 2021-12-01 |
| 页码 | 11 |
| ISSN号 | 1341-8076 |
| 关键词 | female infertility nutrition ovarian function pharmacology postpartum care |
| DOI | 10.1111/jog.15122 |
| 英文摘要 | Aims This study aimed to prepare swim bladder hydrolysate (SBH) with M-n < 4000 Da, and investigate its effects on cyclophosphamide (CTX)-mediated ovarian injury in mice. Methods Hydrolysates were prepared by heating extraction, enzymatic hydrolysis and ultrafiltration. M-n and distribution of SBH were analyzed via gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Changes in the mouse oestrus cycle were determined by cytological examination. The number of follicles was examined using histopathology. Enzyme-linked immunosorbent assays (ELISAs) were used to determine the serum sex hormone levels. Results The M-n of SBH, prepared by heating extraction, enzymatic hydrolysis, ultrafiltration, and from different batches, was below 4000 Da, and the preparation process was stable. Compared with the control group, the low-, middle-, and high-dose SBH treatment groups showed different trends in oestrus duration, serum sex hormone levels, and the number of primordial and secondary follicles. The oestrus cycle duration of the high-dose SBH group was longer than that of the model group. The serum luteinizing hormone, follicle-stimulating hormone, and anti-Mullerian hormone levels in the middle-dose group were the closest to those of control group. The number of primordial and secondary follicles in the medium-dose group was significantly higher than that in the model group and closest to those of control group. Conclusion After heating extraction, trypsin/Flavourzyme hydrolysis and ultrafiltration, a hydrolysate with M-n below 4000 Da could be prepared. We found that a moderate (400 mg/kg) SBH dose resulted in the greatest effect on ovarian injury remission in mice. |
| WOS关键词 | ANTI-MULLERIAN HORMONE ; GRANULOSA-CELLS ; CHEMOTHERAPY ; FAILURE ; WOMEN ; ANTIOXIDANT ; PREMATURE ; TOXICITY ; RESERVE ; PROTEIN |
| 资助项目 | National Natural Science Foundation of China[81770527] ; National Natural Science Foundation of China[82171700] ; Natural Science Foundation of Guangdong[2017A020214020] ; Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi[2020KY12033] |
| WOS研究方向 | Obstetrics & Gynecology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000724446300001 |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Guangdong ; Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/51278]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Liu, Tao |
| 作者单位 | 1.Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China 2.Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 4.Univ South China, Inst Pharm & Pharmacol, Hengyang, Peoples R China 5.Guilin Med Univ, Sch Pharm, Guilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Enhui,Zha, Rui,Gao, Qing,et al. Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice[J]. JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,2021:11. |
| APA | Ge, Enhui.,Zha, Rui.,Gao, Qing.,Guo, Lirong.,Wang, Binglong.,...&Liu, Tao.(2021).Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice.JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,11. |
| MLA | Ge, Enhui,et al."Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice".JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (2021):11. |
入库方式： OAI收割
来源：过程工程研究所
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