Low deacetylation degree chitosan oligosaccharide protects against IL-1 beta induced inflammation and enhances autophagy activity in human chondrocytes
文献类型：期刊论文
| 作者 | Cao, Ruiqi1; Yu, Haomiao1; Long, Huibin1; Zhang, Hongrui1; Hao, Chao1; Shi, Lin1; Du, Yuguang2; Jiao, Siming2; Guo, Ai1; Ma, Lifeng1 |
| 刊名 | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
 |
| 出版日期 | 2021-10-21 |
| 页码 | 15 |
| 关键词 | Osteoarthritis chitosan oligosaccharide degree of deacetylation autophagy chondrocytes inflammation |
| ISSN号 | 0920-5063 |
| DOI | 10.1080/09205063.2021.1996962 |
| 英文摘要 | Osteoarthritis (OA) is a degenerative joint disease, which can lead to joint pain, stiffness, deformity and dysfunction, that seriously affects the quality of life in patients. At present, the treatments of OA mainly include early pharmacological treatment and late joint replacement. However, current pharmacological treatment has limited efficacy and undesired side effects. Chitosan oligosaccharide (COS) is a kind of nontoxic and biodegradable oligo-saccharide, which is composed of 2-20 glucosamine or N-acetylglucosamine linked by beta-1,4 glycosidic bond. Studies have shown that COS has significant biological properties like antimicrobial, anti-inflammatory, antioxidant, and anti-tumor, as well as immunoregulation ability. However, the effects of COS on OA have not been clarified. In this study, we explored the protective effects of COS with different degrees of deacetylation on chondrocytes stimulated by interleukin 1 beta (IL-1 beta) in vitro. The results showed that IL-1 beta inhibited cell proliferation and promoted cell apoptosis. Besides that, IL-1 beta increased the expression of the major chondro-degrading genes MMP13 and ADAMTS-5, while decreased the expression of COL2A and ACAN. COS with different degrees of deacetylation (HDACOS, MDACOS, LDACOS) had different effects on IL-1 beta induced inflammation. LDACOS had the most obvious anti-inflammatory effects to inhibit the expression of MMP13 and ADAMTS-5 while promoted the expression of COL2A and ACAN. In addition, we found that the expression of autophagy-related gene Beclin-1 was up-regulated, and the ratio of LC3-II/LC3-I was increased in the LDACOS group. Furthermore, transmission electron microscopy (TEM) analysis showed that the number of intracellular autophagosomes increased significantly with the treatment of LDACOS. Based on our research, we suggested that LDACOS could inhibit chondrocytes inflammation and promote cell autophagy, and might be a protective drug for the treatment of OA. |
| WOS关键词 | ARTICULAR-CARTILAGE ; MOLECULAR-WEIGHT ; GLOBAL BURDEN ; OSTEOARTHRITIS ; CHITOOLIGOSACCHARIDES ; DEGENERATION ; PATHOGENESIS ; ACTIVATION ; EXPRESSION ; DISEASE |
| 资助项目 | project of Beijing Natural Science Foundation[7192045] |
| WOS研究方向 | Engineering ; Materials Science ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000732595500001 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 资助机构 | project of Beijing Natural Science Foundation |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/51467]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Guo, Ai; Ma, Lifeng; Wang, Zhuo |
| 作者单位 | 1.Capital Med Univ, Beijing Friendship Hosp, Dept Orthoped, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Ruiqi,Yu, Haomiao,Long, Huibin,et al. Low deacetylation degree chitosan oligosaccharide protects against IL-1 beta induced inflammation and enhances autophagy activity in human chondrocytes[J]. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,2021:15. |
| APA | Cao, Ruiqi.,Yu, Haomiao.,Long, Huibin.,Zhang, Hongrui.,Hao, Chao.,...&Wang, Zhuo.(2021).Low deacetylation degree chitosan oligosaccharide protects against IL-1 beta induced inflammation and enhances autophagy activity in human chondrocytes.JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,15. |
| MLA | Cao, Ruiqi,et al."Low deacetylation degree chitosan oligosaccharide protects against IL-1 beta induced inflammation and enhances autophagy activity in human chondrocytes".JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2021):15. |
入库方式： OAI收割
来源：过程工程研究所
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