Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds
文献类型：期刊论文
| 作者 | Tan, Minghui1,2; Zhao, Qingsheng1,2 ; Zhao, Bing1
|
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2021-12-15 |
| 卷号 | 193页码:1635-1644 |
| 关键词 | Chenopodium quinoa polysaccharides Structure Biological activities |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2021.10.226 |
| 英文摘要 | In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds. The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR. Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence. The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively. The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively. All three polysaccharide fractions contained triple-helix structure. Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells. These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food. |
| WOS关键词 | CHEMICAL-CHARACTERIZATION ; ENTEROMORPHA-PROLIFERA ; ANTIOXIDANT ACTIVITIES ; CHAIN CONFORMATION ; ALPHA-GLUCOSIDASE ; EXTRACTION ; PURIFICATION ; LENTINAN |
| 资助项目 | Government of China[HNYH-2017-80] ; Government of China[4TB202007] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000734369500010 |
| 出版者 | ELSEVIER |
| 资助机构 | Government of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/51515]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhao, Qingsheng; Zhao, Bing |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Div Bioresources & Hlth Prod Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Minghui,Zhao, Qingsheng,Zhao, Bing. Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,193:1635-1644. |
| APA | Tan, Minghui,Zhao, Qingsheng,&Zhao, Bing.(2021).Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,193,1635-1644. |
| MLA | Tan, Minghui,et al."Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 193(2021):1635-1644. |
入库方式： OAI收割
来源：过程工程研究所
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