Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes
文献类型：期刊论文
| 作者 | Chu, Hongwei1; Zhao, Qun1; Liu, Ju2; Yang, Kaiguang1; Wang, Yanlei2; Liu, Jianhui1; Zhang, Kun1; Zhao, Baofeng1; He, Hongyan2; Zheng, Yong3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2021-12-21 |
| 页码 | 10 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.1c03195 |
| 英文摘要 | Limited by the rare efficient extraction system in extracting hydrophobic membrane protein complexes (MPCs) without compromising the stability of protein-protein interactions (PPIs), the in-depth functional study of MPCs has lagged far behind. In this study, the first systematic screening of ionic liquids (ILs) was performed and showed that triethylammonium acetate (TEAA) IL exhibited excellent performance in stabilizing PPIs, which was further confirmed by molecular docking simulations. By combining TEAA with the conventional detergent Nonidet P-40 (NP-40), a novel IL-based extraction system, i-TAN (TEAA IL with 1% NP-40), was proposed, which demonstrated superior performance in extracting and stabilizing MPCs, attributed to its larger size, more uniform distribution, and closer-to-neutral microenvironment of micelles. Extraction of MPCs with i-TAN allowed the confident identification of more hydrophobic EGFR-interacting proteins that are easily dissociated during the extraction process. Quantitative analysis of the difference in EGFR complexes between trastuzumab-sensitive and trastuzumab-resistant breast cancer cells provided comprehensive insights to understand the drug resistance mechanism, suggesting that i-TAN has great potential in interactomics and functional analysis of MPCs. This study provides a novel strategy for MPC extraction and downstream processing. |
| WOS关键词 | EGFR ; STABILIZATION ; TRASTUZUMAB ; LANDSCAPE ; STABILITY ; NETWORKS ; COVERAGE ; SINGLE |
| 资助项目 | National Key Research and Development Program of China[2016YFA0501401] ; National Key Research and Development Program of China[2018YFA0507703] ; National Natural Science Foundation[21834006] ; National Natural Science Foundation[21725506] ; National Natural Science Foundation[31770893] ; National Natural Science Foundation[21775150] ; National Natural Science Foundation[21922813] ; CAS Key Project in Frontier Science[QYZDY-SSW-SLH017] ; Youth Innovation Promotion Association, CAS[2017066] ; Youth Innovation Promotion Association, CAS[2020184] ; Cooperation agreement of Innovation Academy for Precision Measurement Science and Technology, CAS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000735943400001 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; Youth Innovation Promotion Association, CAS ; Cooperation agreement of Innovation Academy for Precision Measurement Science and Technology, CAS |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/51528]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhao, Baofeng; He, Hongyan; Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Multiphase Complex Syst, CAS Key Lab Green Proc & Engn,Beijing Key Lab Ion, Beijing 100190, Peoples R China 3.Beijing Inst Life, Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China 4.Dalian Univ Technol, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chu, Hongwei,Zhao, Qun,Liu, Ju,et al. Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes[J]. ANALYTICAL CHEMISTRY,2021:10. |
| APA | Chu, Hongwei.,Zhao, Qun.,Liu, Ju.,Yang, Kaiguang.,Wang, Yanlei.,...&Zhang, Yukui.(2021).Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.ANALYTICAL CHEMISTRY,10. |
| MLA | Chu, Hongwei,et al."Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes".ANALYTICAL CHEMISTRY (2021):10. |
入库方式： OAI收割
来源：过程工程研究所
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