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Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus

文献类型：期刊论文

作者Sheng, Yanan1,2; Wei, Jiangxue1,2; Li, Zhengjun1; Su, Zhiguo1; Ma, Guanghui1; Zhang, Songping1
刊名PARTICUOLOGY
出版日期2022-05-01
卷号64页码:56-64
关键词Influenza vaccine Apoferritin nanoparticle Hemagglutinin M2e Conjugate vaccine
ISSN号1674-2001
DOI10.1016/j.partic.2021.04.001
英文摘要Ferritin nanoparticles with self-assembling properties have been widely explored as vaccine carrier by displaying foreign antigens through genetic fusion strategy. In the present work, an apoferritin (AFt) nanoparticle was tested as influenza vaccine carrier by chemically conjugating a matrix protein 2 ectodomain (M2e) antigen peptide or/and the full-length hemagglutinin (HA) antigen on the outer surface of the AFt, with heterobifunctional sSMCC or SM(PEG)24 containing PEG chain as linkers. To each AFt nanoparticle, about 30-32 M2e or 1.8 HA antigen could be coupled. The AFt-(PEG)(24)-M2e, in which the M2e was coupled through SM(PEG)24 containing PEG chain, conferred higher protective efficacy in immunized mice than AFt-M2e did, but was less effective than AFt-(PEG)(24)-HA. When both M2e and HA were coupled, the synthesized dual-antigen vaccine candidate AFt-(PEG)(24)-M2e/HA elicited high level of M2e and HA antigen-specific antibodies and conferred 100% protection against lethal infection of homologous PR8 H1N1 virus strain and 70% protection against a heterologous A/FM/1/47 (FM1, H1N1) strain, which was more effective than the M2e or HA single antigen vaccine candidates. The potential cross-protective effect of the dual-antigen vaccine was further demonstrated by significant specific hemagglutination inhibition (HAI) titers in serum of the immunized mice against three other heterologous viral strains including A/Singapore/GP1908/2015 (IVR-180) H1N1, A/Anhui/1/2005 H5N1, and A/Hong Kong H3N2. (C) 2021 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V.
资助项目National Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[31970872]
WOS研究方向Engineering ; Materials Science
语种英语
WOS记录号WOS:000743069300006
出版者ELSEVIER SCIENCE INC
资助机构National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/51699]  
专题中国科学院过程工程研究所
通讯作者Zhang, Songping
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Sheng, Yanan,Wei, Jiangxue,Li, Zhengjun,et al. Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus[J]. PARTICUOLOGY,2022,64:56-64.
APA Sheng, Yanan,Wei, Jiangxue,Li, Zhengjun,Su, Zhiguo,Ma, Guanghui,&Zhang, Songping.(2022).Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus.PARTICUOLOGY,64,56-64.
MLA Sheng, Yanan,et al."Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus".PARTICUOLOGY 64(2022):56-64.

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来源：过程工程研究所

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