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O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice

文献类型：期刊论文

作者Shao Wen-Ya1,2; Liang Yu2; Liu Jian-Xi4; Liu Hong-Tao3; Wang Zhao-Wei5; Sui Zhi-Gang2; Zhao Bao-Feng2; Zhang Xiao-Dan2; Liang Zhen2; Zhang Li-Hua2
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
出版日期2021-12-01
卷号49期号:12页码:11
ISSN号0253-3820
关键词O-linked beta-N-acetylglucosaminylation Quantitative proteomics Electron transfer/higher energy collision dissociation
DOI10.19756/j.issn.0253-3820.210623
英文摘要O-linked beta-N-acetylglucosaminylation (O-GlcNAcylation ) is a single carbohydrate moiety post-translational modification that occurs on serine and threonine side chains of intracellular protein and plays an important role in nutrient metabolism. However, the great research challenges are the inherently low stoichiometry, , poor ionization efficiency of O-GlcNAc peptides and no specific animo acid sequence. In this study, a strategy for quantitative detection of O-GlcNAc sites was developed by combining pseudo-isobaric dimethyl strategy, which was applied for the O-GlcNAcylation sites profiling towards the mice liver. In total, 783 O-GlcNAc sites were unambiguously quantified from high-fat fed mice liver. Among which , 122 O-GlcNAc sites were differentially expressed, corresponding to 85 O-GlcNAc proteins. Finally, the biological function of the differentially expressed O-GlcNAc proteins was analyzed.
WOS关键词OGT ; GLYCOPROTEINS ; ACTIVATION
WOS研究方向Chemistry
语种英语
出版者SCIENCE PRESS
WOS记录号WOS:000753629000001
源URL[http://ir.ipe.ac.cn/handle/122111/51976]  
专题中国科学院过程工程研究所
通讯作者Zhang Li-Hua
作者单位1.Fujian Med Univ, Sch Publ Hlth, Dept Prevent Med, Fuzhou 350122, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
4.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Peoples R China
5.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Shao Wen-Ya,Liang Yu,Liu Jian-Xi,et al. O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2021,49(12):11.
APA Shao Wen-Ya.,Liang Yu.,Liu Jian-Xi.,Liu Hong-Tao.,Wang Zhao-Wei.,...&Zhang Yu-Kui.(2021).O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49(12),11.
MLA Shao Wen-Ya,et al."O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49.12(2021):11.

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来源：过程工程研究所

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