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Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging

文献类型：期刊论文

作者Yang, Hongwei1,2; Wu, Yayun2; Ruan, Hefei2; Guo, Feng3; Liang, Yuxin1,2; Qin, Gege1,2; Liu, Xiaolong1,2; Zhang, Zhen2; Yuan, Jinghe2; Fang, Xiaohong2,4
刊名ANALYTICAL CHEMISTRY
出版日期2022-02-10
页码9
ISSN号0003-2700
DOI10.1021/acs.analchem.1c03935
英文摘要Stimulated emission depletion (STED) nanoscopy is an emerging super-resolution imaging platform for the study of the cellular structure. Developing suitable fluorescent probes of small size, good photostability, and easy functionalization is still in demand. Herein, we introduce a new type of surface-engineered gold nanoclusters (Au NCs) that are ultrasmall (1.7 nm) and ultrabright (QY = 60%) for STED bioimaging. A rigid shell formed by L-arginine (L-Arg) and 6-aza-2thiothymine (ATT) on the Au NC surface enables not only its strong fluorescence in aqueous solution but also its easy chemical modification for specific biomolecule labeling. Au NCs show remarkable performance as STED nanoprobes, including high depletion efficiency, good photobleaching resistance, and low saturation intensity. Super-resolution imaging has been achieved with these Au NCs, and targeted nanoscopic imaging of cellular tubulin has been demonstrated. Moreover, the circular structure of lysosomes in live cells has been revealed. As a Au NC is also an ideal probe for electron microscopy, dual imaging of A beta 42 aggregates with the single labeling probe of Au NCs has been realized in correlative light and electron microscopy (CLEM). This work reports, for the first time, the application of Au NCs as a novel probe in STED and CLEM imaging. With their excellent properties, Au NCs show promising potential for nanoscale bioimaging.
WOS关键词SUPERRESOLUTION ; NANOPARTICLES ; FLUORESCENCE ; PROBES
资助项目National Natural Science Foundation of China[21735006] ; National Natural Science Foundation of China[21890742] ; National Natural Science Foundation of China[91939301] ; National Natural Science Foundation of China[22077124] ; Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000757849700001
出版者AMER CHEMICAL SOC
资助机构National Natural Science Foundation of China ; Chinese Academy of Sciences
源URL[http://ir.ipe.ac.cn/handle/122111/51993]  
专题中国科学院过程工程研究所
通讯作者Yuan, Jinghe; Fang, Xiaohong
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Anal & Testing Ctr, Inst Proc Engn, Beijing 100190, Peoples R China
4.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Hongwei,Wu, Yayun,Ruan, Hefei,et al. Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging[J]. ANALYTICAL CHEMISTRY,2022:9.
APA Yang, Hongwei.,Wu, Yayun.,Ruan, Hefei.,Guo, Feng.,Liang, Yuxin.,...&Fang, Xiaohong.(2022).Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging.ANALYTICAL CHEMISTRY,9.
MLA Yang, Hongwei,et al."Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging".ANALYTICAL CHEMISTRY (2022):9.

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来源：过程工程研究所

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