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Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage

文献类型：期刊论文

作者Jiang, Zhen1,2; Wu, Dildar1,3; Liang, Zong-Lin1,2; Li, Xiu-Tong1,2; Huang, Ye1,2; Zhou, Nan1; Liu, Zheng-Hua4; Zhang, Guang-Ji5,6; Jia, Yan5,6; Yin, Hua-Qun4
刊名INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
出版日期2022
卷号72期号:3页码:8
关键词Alicyclobacillus curvatus Alicyclobacillus mengziensis acid mine drainage acidophile
ISSN号1466-5026
DOI10.1099/ijsem.0.005285
英文摘要Two acidophilic strains, designated as ALEF1(T) and S30H14(T), were isolated from acid mine drainage sediment. Cells of both strains were Gram-stain-positive, aerobic, endospore-forming rods. Strains ALEF1(T) and S30H14(T) were acidophilic and mesophilic, the former grew at 20-40 degrees C (optimum, 30 degrees C) and pH 2.5-4.5 (optimum, pH 3.5), while the latter grew at 20-45 degrees C (optimum, 30 degrees C) and pH 2.0-5.5 (optimum, pH 4.5). The 16S rRNA gene-based sequence analysis revealed that strains ALEF1(T) and S30H14(T) belonged to the genus Alicyclobacillus, and were phylogenetically close to Alicyclobacillus ferrooxydans TC-34(T) with 97.1 and 97.4% similarity, respectively. The similarity between the two novel strains was 98.6 %. The average nucleotide identity value between the genome sequences of ALEF1(T) and S30H14(T) was 79.5 %, and that between each of the two isolates and A. ferrooxydans TC-34(T) were 72.0 and 74.3 %. In addition, the digital DNA-DNA hybridization value between ALEF1(T) and S30H14(T) was 24.9 %, between strain ALEF1(T) and A. ferrooxydans TC-34(T) was 21.7 %, and between S30H14(T) and A. ferrooxydans TC-34(T) was 26.3 %, far below the interspecies threshold. Both strains could utilize diverse carbon sources for heterotrophic growth; strain ALEF1(T) could utilize ferrous iron as the energy source for autotrophic growth. Menaquinone 7 was the only quinone detected in either strain. Both strains contained anteiso-C-15:0 and anteiso-C-17:0, while omega-alicyclic fatty acids were not detected. Based on their phylogenetic positions, as well as phenotypic and genomic data, it is considered that strains ALEF1(T) and S30H14(T) represent two novel species within the genus Alicyclobacillus, for which the names Alicyclobacillus curvatus sp. nov. (type strain ALEF1(T)=CGMCC 1.17055(T)=KCTC 43124(T)) and Alicyclobacillus mengziensis sp. nov. (S30H14(T)=CGMCC 1.17050(T)=KCTC 43125(T)) are proposed.
WOS关键词BACILLUS-ACIDOCALDARIUS ; OXIDIZING BACTERIUM ; EMENDED DESCRIPTION ; FATTY-ACIDS ; GENUS ; EXTRACTION
资助项目Major Research Plan of the National Natural Science Foundation of China[91851206] ; CAS Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences[KFJ-PTXM-016] ; CAS-NSTDA Joint Research Program[53211KYSB20200039] ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences[IAGM2020C24] ; National Natural Science Foundation of China[31670124]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000785355900010
出版者MICROBIOLOGY SOC
资助机构Major Research Plan of the National Natural Science Foundation of China ; CAS Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences ; CAS-NSTDA Joint Research Program ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/52881]  
专题中国科学院过程工程研究所
通讯作者Liu, Shuang-Jiang; Jiang, Cheng-Ying
作者单位1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Kashi Univ, Coll Life & Geog Sci, Kashi 844000, Peoples R China
4.Cent South Univ, Sch Minerals Proc & Bioengn, Key Lab Biomet, Minist Educ, Changsha, Peoples R China
5.Chinese Acad Sci, Innovat Acad Green Manufacture, Beijing 100190, Peoples R China
6.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Zhen,Wu, Dildar,Liang, Zong-Lin,et al. Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2022,72(3):8.
APA Jiang, Zhen.,Wu, Dildar.,Liang, Zong-Lin.,Li, Xiu-Tong.,Huang, Ye.,...&Jiang, Cheng-Ying.(2022).Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,72(3),8.
MLA Jiang, Zhen,et al."Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 72.3(2022):8.

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来源：过程工程研究所

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