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From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

文献类型：期刊论文

作者Cao, Weilei1,2; Deng, Tingting3; Cao, Weifeng1,2; Shen, Fei1,2; Wan, Yinhua1,2
刊名SEPARATION AND PURIFICATION TECHNOLOGY
出版日期2022-05-01
卷号288页码:11
ISSN号1383-5866
关键词Fructo-oligosaccharides Membrane Fructosyltransferases Diafiltration
DOI10.1016/j.seppur.2022.120678
英文摘要Fructo-oligosaccharides (FOS) are a group of linear fructose oligomers with numerous health benefits, which can be synthesized by the transfructosylation of sucrose using fructosyltransferases (FTases). In this study, it was found that the selected FTases showed both hydrolytic and transfructosylating activities. For FOS production, the hydrolytic activities of FTases became significant when the reaction time was above 1 h. Thus, FTases needed to be inactivated or removed from the reactor after achieving the highest FOS conversion. We therefore developed an integrated ultrafiltration-diafiltration-concentration process for FTases reuse and FOS purification. Because of the 100% rejection of FTases activity and a higher average flux, a PES-10 ultrafiltration membrane was selected for FTases recovery, where the cake layer formation was the main membrane fouling mechanism. Then, a fed batch reaction process was developed for FOS production, where the hydrolytic behavior of FTases was negligible, and the FTases dosage was reduced by 75% compared to single batch operation (four batches). Moreover, the FOS production was comparable, and 95.5% FTases was recovered by the PES10 membrane after the four fed-batches processes. Subsequently, a NF5 nanofiltration membrane was selected to further separate FOS from the FTases-free FOS solution by a constant volume diafiltration process under 45 ?, and the monosaccharides in the reaction mixture (glucose and fructose) were completely removed, increasing the FOS purity from 55.8% to 92.3%. Finally, the FOS and sucrose in the diafiltration permeate was recovered by a DL nanofiltration membrane under 60 C. The outcome of this work offers a complete technical route for FOS production using sucrose as substrate.
WOS关键词FRUCTOSYLTRANSFERASE ; IMMOBILIZATION ; FERMENTATION ; COCULTURE
资助项目Beijing Natural Science Foundation, China[5182025] ; National Natural Science Foundation of China, China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005] ; Science and Technology Service Network Program of Chinese Academy of Sciences
WOS研究方向Engineering
语种英语
出版者ELSEVIER
WOS记录号WOS:000782997100005
资助机构Beijing Natural Science Foundation, China ; National Natural Science Foundation of China, China ; National High Technology Research and Development Program of China ; Science and Technology Service Network Program of Chinese Academy of Sciences
源URL[http://ir.ipe.ac.cn/handle/122111/52891]  
专题中国科学院过程工程研究所
通讯作者Cao, Weifeng; Wan, Yinhua
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
3.Chinese Acad Inspect & Quarantine, Agroprod Safety Res Ctr, Beijing 100123, Peoples R China
推荐引用方式
GB/T 7714		 Cao, Weilei,Deng, Tingting,Cao, Weifeng,et al.

From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2022,288:11.
APA Cao, Weilei,Deng, Tingting,Cao, Weifeng,Shen, Fei,&Wan, Yinhua.(2022).

From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

.SEPARATION AND PURIFICATION TECHNOLOGY,288,11.
MLA Cao, Weilei,et al."

From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

".SEPARATION AND PURIFICATION TECHNOLOGY 288(2022):11.

入库方式： OAI收割

来源：过程工程研究所

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