Conjugation with loxoribine and mannan improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein
文献类型：期刊论文
| 作者 | Yu, Weili; Shen, Lijuan; Qi, Jinming; Hu, Tao |
| 刊名 | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
 |
| 出版日期 | 2022-03-01 |
| 卷号 | 172页码:193-202 |
| ISSN号 | 0939-6411 |
| 关键词 | Loxoribine Mannan Conjugation Subunit vaccines |
| DOI | 10.1016/j.ejpb.2022.02.011 |
| 英文摘要 | Protein-based subunit vaccines have received great attention due to their high safety and specificity. However, the protein antigens are poorly immunogenic, necessitating the formulation with adjuvants and antigen delivery systems. As a ligand of TLR7/8, loxoribine markedly enhances cellular and humoral immune responses. Mannan, a biocompatible polysaccharide adjuvant, can be recognized by the mannose receptor and DC-SIGN. CFP10-TB10.4 fusion protein (CT) is a recombinant fusion protein antigen of Mycobacterium tuberculosis. In the present study, CT was conjugated with loxoribine and mannan to improve the immunogenicity of CT. The conjugate (CT-man-lox) elicited high CT-specific IgG titers (1.1 x 10(4)) in C57BL/6 mice. Th1-type cytokines (IFN-gamma, TNF-alpha, and IL-2) and Th2-type cytokine (IL-4) were secreted at high levels. Moreover, CT-man-lox stimulated the splenocyte proliferation and enhanced the CD3(+), CD4(+) and CD8(+) T cell populations. Pharmacokinetics suggested that conjugation with loxoribine and mannan prolonged the in vivo serum duration of CT. Pharmacodynamics indicated that CT-man-lox elicited an intense production of CT-specific IgG. Thus, conjugation with loxoribine and mannan additively stimulated strong cellular and humoral immune response to CT. CT-man-lox was expected to act an effective protein-based vaccine against Mycobacterium tuberculosis. |
| WOS关键词 | DELIVERY-SYSTEM ; VACCINE ; CONJUGATION ; ADJUVANT ; FORMULATION ; AGONIST ; TLR7 ; IMMUNITY ; ANTIGEN ; DESIGN |
| 资助项目 | National Natural Science Foundation of China[31970875] ; Beijing Natural Science Foundation of China[M21013] ; National Key Research and Development Project of China[2018YFA0900804] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000788072500003 |
| 资助机构 | National Natural Science Foundation of China ; Beijing Natural Science Foundation of China ; National Key Research and Development Project of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/52976]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Qi, Jinming; Hu, Tao |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Yu, Weili,Shen, Lijuan,Qi, Jinming,et al. Conjugation with loxoribine and mannan improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein [J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2022,172:193-202. |
| APA |
Yu, Weili,Shen, Lijuan,Qi, Jinming,&Hu, Tao.(2022). Conjugation with loxoribine and mannan improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein .EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,172,193-202. |
| MLA |
Yu, Weili,et al." Conjugation with loxoribine and mannan improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein ".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 172(2022):193-202. |
入库方式： OAI收割
来源：过程工程研究所
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