Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants
文献类型：期刊论文
| 作者 | Fan, Da-Yong; Fitzpatrick, Duncan2; Oguchi, Riichi2,5; Ma, Weimin2,4; Kou, Jiancun2,3; Chow, Wah Soon2 |
| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 129期号:3 |
| ISSN号 | 0166-8595 |
| 关键词 | Cyclic electron flux Linear electron flux Membrane inlet mass spectrometry P700 Photosystem I Photosystem II |
| DOI | 10.1007/s11120-016-0223-4 |
| 文献子类 | Review |
| 英文摘要 | Sixty years ago Arnon and co-workers discovered photophosphorylation driven by a cyclic electron flux (CEF) around Photosystem I. Since then understanding the physiological roles and the regulation of CEF has progressed, mainly via genetic approaches. One basic problem remains, however: quantifying CEF in the absence of a net product. Quantification of CEF under physiological conditions is a crucial prerequisite for investigating the physiological roles of CEF. Here we summarize current progress in methods of CEF quantification in leaves and, in some cases, in isolated thylakoids, of C3 plants. Evidently, all present methods have their own shortcomings. We conclude that to quantify CEF in vivo, the best way currently is to measure the electron flux through PS I (ETR1) and that through PS II and PS I in series (ETR2) for the whole leaf tissue under identical conditions. The difference between ETR1 and ETR2 is an upper estimate of CEF, mainly consisting, in C3 plants, of a major PGR5-PGRL1-dependent CEF component and a minor chloroplast NDH-dependent component, where PGR5 stands for Proton Gradient Regulation 5 protein, PGRL1 for PGR5-like photosynthesis phenotype 1, and NDH for Chloroplast NADH dehydrogenase-like complex. These two CEF components can be separated by the use of antimycin A to inhibit the former (major) component. Membrane inlet mass spectrometry utilizing stable oxygen isotopes provides a reliable estimation of ETR2, whilst ETR1 can be estimated from a method based on the photochemical yield of PS I, Y(I). However, some issues for the recommended method remain unresolved. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1573-5079 |
| 出版地 | DORDRECHT |
| WOS关键词 | WATER-WATER CYCLE ; INDUCED ABSORBENCY CHANGES ; EXCESS EXCITATION-ENERGY ; THYLAKOID MEMBRANES ; QUANTUM YIELD ; CHLAMYDOMONAS-REINHARDTII ; REDOX STATE ; C-3 PLANTS ; 820 NM ; FLOW |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000383584600003 |
| 资助机构 | Australian Research CouncilAustralian Research Council [DP1093827] ; Knowledge Innovation Program of the Chinese Academy of SciencesChinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-XB3-09-02] ; NNSF of ChinaNational Natural Science Foundation of China (NSFC) [31370424] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/24504]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Australian Natl Univ, Res Sch Biol, Div Plant Sci, 46 Sullivans Creek Rd, Acton, ACT 2601, Australia 2.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 3.Shanghai Normal Univ, Coll Life & Environm Sci, Guilin Rd 100, Shanghai 200234, Peoples R China 4.Tohoku Univ, Grad Sch Life Sci, Sendai, Miyagi 9808578, Japan 5.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Da-Yong,Fitzpatrick, Duncan,Oguchi, Riichi,et al. Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants[J]. PHOTOSYNTHESIS RESEARCH,2016,129(3). |
| APA | Fan, Da-Yong,Fitzpatrick, Duncan,Oguchi, Riichi,Ma, Weimin,Kou, Jiancun,&Chow, Wah Soon.(2016).Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants.PHOTOSYNTHESIS RESEARCH,129(3). |
| MLA | Fan, Da-Yong,et al."Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants".PHOTOSYNTHESIS RESEARCH 129.3(2016). |
入库方式： OAI收割
来源：植物研究所
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