RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway
文献类型：期刊论文
| 作者 | Chan, Zhulong; Wang, Yanping; Cao, Minjie; Gong, Yuehua1; Mu, Zixin10; Wang, Haiqing9; Hu, Yuanlei8; Deng, Xin7; He, Xin-Jian; Zhu, Jian-Kang |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2016 |
| 卷号 | 209期号:4 |
| ISSN号 | 0028-646X |
| 关键词 | C-REPEAT-BINDING FACTOR (CBF) cold Pol II RNA-directed DNA methylation RNA-DIRECTED DNA METHYLATION 4 (RDM4) transcriptome |
| DOI | 10.1111/nph.13727 |
| 文献子类 | Article |
| 英文摘要 | The C-REPEAT-BINDING FACTOR (CBF) pathway has important roles in plant responses to cold stress. How the CBF genes themselves are activated after cold acclimation remains poorly understood. In this study, we characterized cold tolerance of null mutant of RNA-DIRECTED DNA METHYLATION 4 (RDM4), which encodes a protein that associates with RNA polymerases Pol V and Pol II, and is required for RNA-directed DNA methylation (RdDM) in Arabidopsis. The results showed that dysfunction of RDM4 reduced cold tolerance, as evidenced by decreased survival and increased electrolyte leakage. Mutation of RDM4 resulted in extensive transcriptomic reprogramming. CBFs and CBF regulon genes were down-regulated in rdm4 but not nrpe1 (the largest subunit of PolV) mutants, suggesting that the role of RDM4 in cold stress responses is independent of the RdDM pathway. Overexpression of RDM4 constitutively increased the expression of CBFs and regulon genes and decreased cold-induced membrane injury. A great proportion of genes affected by rdm4 overlapped with those affected by CBFs. Chromatin immunoprecipitation results suggested that RDM4 is important for Pol II occupancy at the promoters of CBF2 and CBF3. We present evidence of a considerable role for RDM4 in regulating gene expression at low temperature, including the CBF pathway in Arabidopsis. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1469-8137 |
| 出版地 | HOBOKEN |
| WOS关键词 | RESPONSIVE GENE-EXPRESSION ; LOW-TEMPERATURE ; TRANSCRIPTION FACTORS ; FREEZING TOLERANCE ; OXIDATIVE STRESS ; CIRCADIAN CLOCK ; ACCLIMATION ; THALIANA ; BINDING ; ROLES |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000373379800022 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370302] ; Knowledge Innovative Key Program of the Chinese Academy of Sciences [54Y154761O01076, Y329631O0263] ; Chinese Academy of SciencesChinese Academy of Sciences ; US National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM059138] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM059138, R01GM070795] Funding Source: NIH RePORTER |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/24733]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA 2.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei Province, Peoples R China 5.Natl Inst Biol Sci, Beijing 102206, Peoples R China 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 7.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 8.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 9.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 10.Yibin Univ, Coll Life Sci & Food Engn, Yibin 644000, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chan, Zhulong,Wang, Yanping,Cao, Minjie,et al. RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway[J]. NEW PHYTOLOGIST,2016,209(4). |
| APA | Chan, Zhulong.,Wang, Yanping.,Cao, Minjie.,Gong, Yuehua.,Mu, Zixin.,...&Zhu, Jian-Kang.(2016).RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway.NEW PHYTOLOGIST,209(4). |
| MLA | Chan, Zhulong,et al."RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway".NEW PHYTOLOGIST 209.4(2016). |
入库方式： OAI收割
来源：植物研究所
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