Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo-Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1
文献类型：期刊论文
| 作者 | Ma, Lin; Tian, Tian; Lin, Rongcheng4; Deng, Xing-Wang; Wang, Haiyang2; Li, Gang |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2016 |
| 卷号 | 9期号:4 |
| ISSN号 | 1674-2052 |
| 关键词 | Light signal FHY3 FAR1 myo-inositol MIPS SA oxidative stress cell death |
| DOI | 10.1016/j.molp.2015.12.013 |
| 文献子类 | Article |
| 英文摘要 | myo-Inositol-1-phosphate synthase (MIPS) catalyzes the limiting step of inositol biosynthesis and has crucial roles in plant growth and development. In response to stress, the transcription of MIPS1 is induced and the biosynthesis of inositol or inositol derivatives is promoted by unknown mechanisms. Here, we found that the light signaling protein FAR-RED ELONGATED HYPOCOTYL3 (FHY3) and its homolog FAR-RED IMPAIRED RESPONSE1 (FAR1) regulate light-induced inositol biosynthesis and oxidative stress responses by activating the transcription of MIPS1. Disruption of FHY3 and FAR1 caused light-induced cell death after dark-light transition, precocious leaf senescence, and increased sensitivity to oxidative stress. Reduction of salicylic acid (SA) accumulation by overexpression of SALICYLIC ACID 3-HYDROXYLASE largely suppressed the cell death phenotype of fhy3 far1 mutant plants, suggesting that FHY3-and FAR1-mediated cell death is dependent on SA. Furthermore, comparative analysis of chromatin immunoprecipitation sequencing and microarray results revealed that FHY3 and FAR1 directly target both MIPS1 and MIPS2. The fhy3 far1 mutant plants showed severely decreased MIPS1/2 transcript levels and reduced inositol levels. Conversely, constitutive expression of MIPS1 partially rescued the inositol contents, caused reduced transcript levels of SA-biosynthesis genes, and prevented oxidative stress in fhy3 far1. Taken together, our results indicate that the light signaling proteins FHY3 and FAR1 directly bind the promoter of MIPS1 to activate its expression and thereby promote inositol biosynthesis to prevent light-induced oxidative stress and SA-dependent cell death. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 电子版国际标准刊号 | 1752-9867 |
| 出版地 | CAMBRIDGE |
| WOS关键词 | RED ELONGATED HYPOCOTYL3 ; CELL-DEATH ; SACCHAROMYCES-CEREVISIAE ; CHLOROPHYLL BIOSYNTHESIS ; INOSITOL METABOLISM ; HYDROGEN-PEROXIDE ; CIRCADIAN CLOCK ; PHYTOCHROME-A ; ACID ; GENE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000373742700007 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31271309] ; 1000-Talents Plan for young researchers of China ; Outstanding Young Scientist Award Fund [JQ201208] ; Taishan Scholar Program of Shandong Provincial Government |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/24670]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 2.Peking Univ, Coll Life Sci, Peking Tsinghua Ctr Life Sci, Natl Lab Prot & Plant Gene Res,Peking Yale Joint, Beijing 100871, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Lin,Tian, Tian,Lin, Rongcheng,et al. Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo-Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1[J]. MOLECULAR PLANT,2016,9(4). |
| APA | Ma, Lin,Tian, Tian,Lin, Rongcheng,Deng, Xing-Wang,Wang, Haiyang,&Li, Gang.(2016).Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo-Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1.MOLECULAR PLANT,9(4). |
| MLA | Ma, Lin,et al."Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo-Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1".MOLECULAR PLANT 9.4(2016). |
入库方式： OAI收割
来源：植物研究所
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