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The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant

文献类型：期刊论文

作者Sun, Li4; Wang, Dandan4; Yin, Zhiqiu4; Zhang, Chengsheng3; Bible, Amber2; Xie, Zhihong1,4
刊名FRONTIERS IN MICROBIOLOGY
出版日期2021-11-19
卷号12页码:13
关键词Azorhizobium caulinodans ORS571 transcriptional regulator flagella exopolysaccharide biofilm symbiosis
DOI10.3389/fmicb.2021.744268
通讯作者Zhang, Chengsheng(zhangchengsheng@caas.cn) ; Xie, Zhihong(zhihongxe211@163.com)
英文摘要Bacterial signal transduction pathways are important for a variety of adaptive responses to environment, such as two-component systems (TCSs). In this paper, we reported the characterization of a transcriptional regulator in Azorhizobium caulinodans ORS571, ActR, with an N-terminal receiver domain and one C-terminal OmpR/PhoB-type DNA binding domain. Sequence analysis showed that ActR shared a high similarity with FtcR regulator of Brucella melitensis 16M known to be involved in flagellar regulation. The structural gene of this regulator was largely distributed in Alphaproteobacteria, in particular in Rhizobiales and Rhodobacterales, and was located within clusters of genes related to motility functions. Furthermore, we studied the biological function of ActR in A. caulinodans grown at the free-living state or in association with Sesbania rostrata by constructing actR gene deletion mutant. In the free-living state, the bacterial flagellum and motility ability were entirely deleted, the expression of flagellar genes was downregulated; and the exopolysaccharide production, biofilm formation, and cell flocculation decreased significantly compared with those of the wild-type strain. In the symbiotic state, Delta actR mutant strain showed weakly competitive colonization and nodulation on the host plant. These results illustrated that FtcR-like regulator in A. caulinodans is involved in flagellar biosynthesis and provide bacteria with an effective competitive nodulation for symbiosis. These findings improved our knowledge of FtcR-like transcriptional regulator in A. caulinodans.
WOS关键词NITROGEN-FIXATION ; ESCHERICHIA-COLI ; FLAGELLA ; SYSTEM ; LIPOPOLYSACCHARIDE ; EXOPOLYSACCHARIDES ; POLYSACCHARIDE ; AZOSPIRILLUM ; CHEMOTAXIS ; REGULATOR
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000726360400001
源URL[http://ir.yic.ac.cn/handle/133337/30938]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者Zhang, Chengsheng; Xie, Zhihong
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China
2.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA
3.Chinese Acad Agr Sci, Tobacco Res Inst, Marine Agr Res Ctr, Qingdao, Peoples R China
4.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Li,Wang, Dandan,Yin, Zhiqiu,et al. The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant[J]. FRONTIERS IN MICROBIOLOGY,2021,12:13.
APA Sun, Li,Wang, Dandan,Yin, Zhiqiu,Zhang, Chengsheng,Bible, Amber,&Xie, Zhihong.(2021).The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant.FRONTIERS IN MICROBIOLOGY,12,13.
MLA Sun, Li,et al."The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant".FRONTIERS IN MICROBIOLOGY 12(2021):13.

入库方式： OAI收割

来源：烟台海岸带研究所

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