中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 地质与地球物理研究所
  3. 中国科学院地质与地球物理研究所
  4. 中国科学院地球与行星物理重点实验室
A Novel Magnetotactic Alphaproteobacterium Producing Intracellular Magnetite and Calcium-Bearing Minerals

文献类型：期刊论文

作者Liu, Peiyu1,5,6; Liu, Yan1,5,6; Ren, Xinyi2,3; Zhang, Zhifei2,3; Zhao, Xiang4; Roberts, Andrew P.4; Pan, Yongxin1,6; Li, Jinhua1,5,6
刊名APPLIED AND ENVIRONMENTAL MICROBIOLOGY
出版日期2021-12-01
卷号87期号:23页码:15
关键词magnetotactic bacteria biomineralization magnetosome calcium phosphate calcium carbonate
ISSN号0099-2240
DOI10.1128/AEM.01556-21
英文摘要Magnetotactic bacteria (MTB) are prokaryotes that form intracellular magnetite (Fe3O4) or greigite (Fe3S4) nanocrystals with tailored sizes, often in chain configurations. Such magnetic particles are each surrounded by a lipid bilayer membrane, called a magnetosome, and provide a model system for studying the formation and function of specialized internal structures in prokaryotes. Using fluorescence-coupled scanning electron microscopy, we identified a novel magnetotactic spirillum, XQGS-1, from freshwater Xingqinggong Lake, Xi'an City, Shaanxi Province, China. Phylogenetic analyses based on 16S rRNA gene sequences indicate that strain XQGS-1 represents a novel genus of the Alphaproteobacteria class in the Proteobacteria phylum. Transmission electron microscopy analyses reveal that strain XQGS-1 forms on average 17 +/- 3 magnetite magnetosome particles with an ideal truncated octahedral morphology, with an average length and width of 88.3 +/- 11.7 nm and 83.3 +/- 11.0 nm, respectively. They are tightly organized into a single chain along the cell long axis close to the concave side of the cell. Intrachain magnetic interactions likely result in these large equidimensional magnetite crystals behaving as magnetically stable single-domain particles that enable bacterial magnetotaxis. Combined structural and chemical analyses demonstrate that XQGS-1 cells also biomineralize intracellular amorphous calcium phosphate (2 to 3 granules per cell; 90.5- +/- 19.3-nm average size) and weakly crystalline calcium carbonate (2 to 3 granules per cell; 100.4- +/- 21.4-nm average size) in addition to magnetite. Our results expand the taxonomic diversity of MTB and provide evidence for intracellular calcium phosphate biomineralization in MTB. IMPORTANCE Biomineralization is a widespread process in eukaryotes that form shells, teeth, or bones. It also occurs commonly in prokaryotes, resulting in more than 60 known minerals formed by different bacteria under wide-ranging conditions. Among them, magnetotactic bacteria (MTB) are remarkable because they might represent the earliest organisms that biomineralize intracellular magnetic iron minerals (i.e., magnetite (Fe3O4] or greigite [Fe3S4]). Here, we report a novel magnetotactic spirillum (XQGS-1) that is phylogenetically affiliated with the Alphaproteobacteria class. In addition to magnetite crystals, XQGS-1 cells form intracellular submicrometer calcium carbonate and calcium phosphate granules. This finding supports the view that MTB are also an important microbial group for intracellular calcium carbonate and calcium phosphate biomineralization.
WOS关键词TRANSMISSION ELECTRON-MICROSCOPY ; AQUASPIRILLUM-MAGNETOTACTICUM ; PHYLOGENETIC ANALYSIS ; SIZE DISTRIBUTIONS ; SP-NOV ; BACTERIA ; MAGNETOSOME ; BIOMINERALIZATION ; MARINE ; FOSSILIZATION
资助项目National Natural Science Foundation of China[41920104009] ; National Natural Science Foundation of China[41890843] ; National Natural Science Foundation of China[41621004] ; National Natural Science Foundation of China[41425008] ; Center for Ocean Mega-Science, Chinese Academy of Sciences[RVKEXUE2019GZ06] ; Australian Research Council[DP160100805] ; Australian Research Council[DP200100765]
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
WOS记录号WOS:000716998300016
出版者AMER SOC MICROBIOLOGY
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Australian Research Council ; Australian Research Council ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Australian Research Council ; Australian Research Council ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Australian Research Council ; Australian Research Council ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Center for Ocean Mega-Science, Chinese Academy of Sciences ; Australian Research Council ; Australian Research Council
源URL[http://ir.iggcas.ac.cn/handle/132A11/103822]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Li, Jinhua
作者单位1.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
2.Northwest Univ, State Key Lab Continental Dynam, Shaanxi Key Lab Early Life & Environm, Xian, Peoples R China
3.Northwest Univ, Dept Geol, Xian, Peoples R China
4.Australian Natl Univ, Res Sch Earth Sci, Canberra, ACT, Australia
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Peoples R China
6.Chinese Acad Sci, Innovat Acad Earth Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Peiyu,Liu, Yan,Ren, Xinyi,et al. A Novel Magnetotactic Alphaproteobacterium Producing Intracellular Magnetite and Calcium-Bearing Minerals[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2021,87(23):15.
APA Liu, Peiyu.,Liu, Yan.,Ren, Xinyi.,Zhang, Zhifei.,Zhao, Xiang.,...&Li, Jinhua.(2021).A Novel Magnetotactic Alphaproteobacterium Producing Intracellular Magnetite and Calcium-Bearing Minerals.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,87(23),15.
MLA Liu, Peiyu,et al."A Novel Magnetotactic Alphaproteobacterium Producing Intracellular Magnetite and Calcium-Bearing Minerals".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 87.23(2021):15.

入库方式： OAI收割

来源：地质与地球物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。