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A universal constructing method for high performance DNA biosensors based on the optimized photoelectrode material and dual recycling amplification

文献类型：期刊论文

作者Chen, Delun2,3; Dong, Fan3; Zou, Xue3; Qiao, Bin2; Wang, Xiaohong3; Zhang, Kexi3; Tu, Jinchun3; Zhen, Chao4; Wu, Qiang2; Xiao, Dan1,5
刊名APPLIED SURFACE SCIENCE
出版日期2022-05-30
卷号585页码:9
关键词Photoelectrochemical DNA sensors Dual recycling amplification Optimization of photoelectrode material Universal method Signal amplification
ISSN号0169-4332
DOI10.1016/j.apsusc.2022.152661
通讯作者Tu, Jinchun(tujinchun@hainanu.edu.cn) ; Zhen, Chao(czhen@imr.ac.cn) ; Wu, Qiang(wuqiang001001@aliyun.com)
英文摘要Many effective strategies for photoelectrochemical DNA sensors have been developed in the past decades. Most of them focused on the detection performance including sensitivity, limit of detection and linear range, but few on the pivotal roles of photoelectrode materials and the universality for strategies. Herein, the photoelectrode material was optimized by density functional theory for improving the comprehensive performances of DNA biosensor for the first time. And the universal strategies, based on dual recycling amplification, was discussed by different nucleic acid sequences. After systematical optimization of detection conditions, a large linear range of 10(-16) to 10(-6) M, high sensitivity of 5.66 mu A & BULL;cm(-2)& BULL;dec(-1) and low limit of detection of 10(-18) M (a gene copy per microliter) were achieved for the detection of DNA sequences from the I27L gene. Finally, detection of DNA sequences from Orientia tsutsugamushi was used to prove the universality of the novel method.
资助项目Hainan Province Clinical Medical Center ; National Natural Science Foundation of China[51762012] ; National Natural Science Foundation of China[51862006] ; National Natural Science Foundation of China[81860373] ; National Natural Science Foundation of China[81902154] ; National Natural Science Foundation of China[82060386] ; National Natural Science Foundation of China[52072377] ; Finance Science and Technology Project of Hainan Province[ZDYF2019161] ; Finance Science and Technology Project of Hainan Province[ZDYF2018106] ; Finance Science and Technology Project of Hainan Province[2019RC221] ; Finance Science and Technology Project of Hainan Province[820RC647] ; CAMS Innovation Fund for Medical Sciences[2019-I2M-5-023] ; Key Laboratory Open Project Fund of Emergency and Trauma of Ministry of Education[KLET-202008] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2020192]
WOS研究方向Chemistry ; Materials Science ; Physics
语种英语
WOS记录号WOS:000776625400003
出版者ELSEVIER
资助机构Hainan Province Clinical Medical Center ; National Natural Science Foundation of China ; Finance Science and Technology Project of Hainan Province ; CAMS Innovation Fund for Medical Sciences ; Key Laboratory Open Project Fund of Emergency and Trauma of Ministry of Education ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
源URL[http://ir.imr.ac.cn/handle/321006/172761]  
专题金属研究所_中国科学院金属研究所
通讯作者Tu, Jinchun; Zhen, Chao; Wu, Qiang
作者单位1.Sichuan Univ, Coll Chem, Key Lab Green & Technol, Minist Educ, Chengdu 610065, Peoples R China
2.Hainan Med Univ, Affiliated Hosp 2, Chinese Acad Med Sci, Sch Trop Med & Lab Med,Dept Clin Lab,Key Lab Emer, Haikou 571199, Hainan, Peoples R China
3.Hainan Univ, Coll Mat Sci & Engn, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
4.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Peoples R China
5.Sichuan Univ, Coll Chem Engn, Chengdu 610065, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Delun,Dong, Fan,Zou, Xue,et al. A universal constructing method for high performance DNA biosensors based on the optimized photoelectrode material and dual recycling amplification[J]. APPLIED SURFACE SCIENCE,2022,585:9.
APA Chen, Delun.,Dong, Fan.,Zou, Xue.,Qiao, Bin.,Wang, Xiaohong.,...&Xiao, Dan.(2022).A universal constructing method for high performance DNA biosensors based on the optimized photoelectrode material and dual recycling amplification.APPLIED SURFACE SCIENCE,585,9.
MLA Chen, Delun,et al."A universal constructing method for high performance DNA biosensors based on the optimized photoelectrode material and dual recycling amplification".APPLIED SURFACE SCIENCE 585(2022):9.

入库方式： OAI收割

来源：金属研究所

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