中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

文献类型:期刊论文

作者Ma, Ying; Ma, Lin; Zheng, Juanjuan; Liu, Min; Zalevsky, Zeev; Gao, Peng
刊名FRONTIERS IN PHYSICS
出版日期2022-05-09
卷号10
关键词quantitative phase imaging phase contrast ultra-oblique illumination label-free non-invasive
ISSN号2296-424X
DOI10.3389/fphy.2022.892529
产权排序3
英文摘要Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system's structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, pi/2, pi, and 3 pi/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.
语种英语
WOS记录号WOS:000803908000001
出版者FRONTIERS MEDIA SA
源URL[http://ir.opt.ac.cn/handle/181661/95997]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
推荐引用方式
GB/T 7714
Ma, Ying,Ma, Lin,Zheng, Juanjuan,et al. High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy[J]. FRONTIERS IN PHYSICS,2022,10.
APA Ma, Ying,Ma, Lin,Zheng, Juanjuan,Liu, Min,Zalevsky, Zeev,&Gao, Peng.(2022).High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy.FRONTIERS IN PHYSICS,10.
MLA Ma, Ying,et al."High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy".FRONTIERS IN PHYSICS 10(2022).

入库方式: OAI收割

来源:西安光学精密机械研究所

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