High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy
文献类型:期刊论文
| 作者 | Ma, Ying; Ma, Lin; Zheng, Juanjuan ; Liu, Min; Zalevsky, Zeev; Gao, Peng
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| 刊名 | FRONTIERS IN PHYSICS
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| 出版日期 | 2022-05-09 |
| 卷号 | 10 |
| 关键词 | quantitative phase imaging phase contrast ultra-oblique illumination label-free non-invasive |
| ISSN号 | 2296-424X |
| DOI | 10.3389/fphy.2022.892529 |
| 产权排序 | 3 |
| 英文摘要 | Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system's structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, pi/2, pi, and 3 pi/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc. |
| 语种 | 英语 |
| WOS记录号 | WOS:000803908000001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.opt.ac.cn/handle/181661/95997] ![]() |
| 专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Ma, Ying,Ma, Lin,Zheng, Juanjuan,et al. High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy[J]. FRONTIERS IN PHYSICS,2022,10. |
| APA | Ma, Ying,Ma, Lin,Zheng, Juanjuan,Liu, Min,Zalevsky, Zeev,&Gao, Peng.(2022).High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy.FRONTIERS IN PHYSICS,10. |
| MLA | Ma, Ying,et al."High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy".FRONTIERS IN PHYSICS 10(2022). |
入库方式: OAI收割
来源:西安光学精密机械研究所
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