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Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)

文献类型：期刊论文

作者Guo, Yan-Yan; Huang, Lai-Qiang; Liu, Zhong-Jian; Wang, Xiao-Quan
刊名PLOS ONE
出版日期2016
卷号11期号:1
ISSN号1932-6203
DOI10.1371/journal.pone.0146880
文献子类Article
英文摘要Orchidaceae are one of the largest families of flowering plants, with over 27,000 species described and all orchids are listed in CITES. Moreover, the seedlings of orchid species from the same genus are similar. The objective of DNA barcoding is rapid, accurate, and automated species identification, which may be used to identify illegally traded endangered species from vegetative specimens of Paphiopedilum (Venus slipper), a flagship group for plant conservation with high ornamental and commercial values. Here, we selected eight chloroplast barcodes and nrITS to evaluate their suitability in Venus slippers. The results indicate that all tested barcodes had no barcoding gap and the core plant barcodes showed low resolution for the identification of Venus slippers (18.86%). Of the single-locus barcodes, nrITS is the most efficient for the species identification of the genus (52.27%), whereas matK + atpF-atpH is the most efficient multi-locus combination (28.97%). Therefore, we recommend the combination of matK + atpF-atpH + ITS as a barcode for Venus slippers. Furthermore, there is an upper limit of resolution of the candidate barcodes, and only half of the taxa with multiple samples were identified successfully. The low efficiency of these candidate barcodes in Venus slippers may be caused by relatively recent speciation, the upper limit of the barcodes, and/or the sampling density. Although the discriminatory power is relatively low, DNA barcoding may be a promising tool to identify species involved in illegal trade, which has broad applications and is valuable for orchid conservation.
学科主题Multidisciplinary Sciences
出版地SAN FRANCISCO
WOS关键词LAND PLANTS ; UNIVERSAL BARCODE ; IDENTIFICATION ; L. ; ORCHIDACEAE ; LOCI ; DISCRIMINATE ; EVOLUTION ; TAXONOMY ; REGIONS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000367888100172
出版者PUBLIC LIBRARY SCIENCE
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30730010, 31300179] ; Chinese Academy of Sciences (the 100-Talent Project) ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2014M550712]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25072]  
专题植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文
作者单位1.Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen, Peoples R China
2.Orchid Conservat & Res Ctr Shenzhen, Shenzhen, Peoples R China
3.Tsinghua Univ, Grad Sch Shenzhen, Ctr Biotechnol & BioMed, Shenzhen 518057, Peoples R China
4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China
5.[Guo, Yan-Yan
推荐引用方式
GB/T 7714		 Guo, Yan-Yan,Huang, Lai-Qiang,Liu, Zhong-Jian,et al. Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)[J]. PLOS ONE,2016,11(1).
APA Guo, Yan-Yan,Huang, Lai-Qiang,Liu, Zhong-Jian,&Wang, Xiao-Quan.(2016).Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum).PLOS ONE,11(1).
MLA Guo, Yan-Yan,et al."Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)".PLOS ONE 11.1(2016).

入库方式： OAI收割

来源：植物研究所

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