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Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity
文献类型：期刊论文
| 作者 | Cheng, Tao; Xu, Chao; Lei, Li1; Li, Changhao; Zhang, Yu3; Zhou, Shiliang |
| 刊名 | MOLECULAR ECOLOGY RESOURCES
 |
| 出版日期 | 2016 |
| 卷号 | 16期号:1页码:138-149 |
| 关键词 | barcoding internal transcribed spacer PCR plant-specific primer |
| ISSN号 | 1755-098X |
| DOI | 10.1111/1755-0998.12438 |
| 文献子类 | Article |
| 英文摘要 | The internal transcribed spacer (ITS) of nuclear ribosomal DNA is one of the most commonly used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been recommended as a core plant DNA barcode. Despite this popularity, the universality and specificity of PCR primers for the ITS region are not satisfactory, resulting in amplification and sequencing difficulties. By thoroughly surveying and analysing the 18S, 5.8S and 26S sequences of Plantae and Fungi from GenBank, we designed new universal and plant-specific PCR primers for amplifying the whole ITS region and a part of it (ITS1 or ITS2) of plants. In silico analyses of the new and the existing ITS primers based on these highly representative data sets indicated that (i) the newly designed universal primers are suitable for over 95% of plants in most groups; and (ii) the plant-specific primers are suitable for over 85% of plants in most groups without amplification of fungi. A total of 335 samples from 219 angiosperm families, 11 gymnosperm families, 24 fern and lycophyte families, 16 moss families and 17 fungus families were used to test the performances of these primers. In vitro PCR produced similar results to those from the in silico analyses. Our new primer pairs gave PCR improvements up to 30% compared with common-used ones. The new universal ITS primers will find wide application in both plant and fungal biology, and the new plant-specific ITS primers will, by eliminating PCR amplification of nonplant templates, significantly improve the quality of ITS sequence information collections in plant molecular systematics and DNA barcoding. |
| 学科主题 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1755-0998 |
| WOS关键词 | INTERNAL TRANSCRIBED SPACER ; DNA BARCODE ; RIBOSOMAL DNA ; ITS2 REGION ; IDENTIFICATION ; PHYLOGENY ; AUTHENTICATION ; DIVERSITY ; MARKERS ; DESIGN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000369141000015 |
| 出版者 | WILEY |
| 资助机构 | Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2011FY120200, 2012AA021602, 2012BAC01B05] ; NSFCNational Natural Science Foundation of China (NSFC) [31270239] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25075]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Beijing Bot Gardens, Beijing 100093, Peoples R China 3.Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA |
| 推荐引用方式 GB/T 7714 | Cheng, Tao,Xu, Chao,Lei, Li,et al. Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity[J]. MOLECULAR ECOLOGY RESOURCES,2016,16(1):138-149. |
| APA | Cheng, Tao,Xu, Chao,Lei, Li,Li, Changhao,Zhang, Yu,&Zhou, Shiliang.(2016).Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity.MOLECULAR ECOLOGY RESOURCES,16(1),138-149. |
| MLA | Cheng, Tao,et al."Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity".MOLECULAR ECOLOGY RESOURCES 16.1(2016):138-149. |
入库方式： OAI收割
来源：植物研究所
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