中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil

文献类型:期刊论文

作者Fu, Shaobin; Li, Yi; Meng, Qingfeng1; Yue, Changwu5; Sun, Yongzhen4; Wei, Gang3
刊名JOURNAL OF THE CHINESE CHEMICAL SOCIETY
出版日期2016
卷号63期号:4页码:331-335
关键词Biocatalysis Region-stereoselectivity Soil microbes Natural product Condition optimization
ISSN号0009-4536
DOI10.1002/jccs.201500501
文献子类Article
英文摘要Oxysophoridine (OSR), an alkaloid extracted from a traditional Chinese medicine, attracted more and more attention because of remarkable biological activities. Biocatalysis of OSR by 86 stains including soil microbes and endophytic fungi were investigated. Incubation of OSR with soil bacteria T003 identified as Escherichia coli could lead to only one reductive product with highly regio-selectivity. The structural elucidations of transformation product were achieved mainly by the NMR spectroscopic methods and references. Effect of pH, substrate concentration, conversion time and strain concentration was studied, the optimization of reaction was analyzed by LC-MS. The production of sophoridine (SR) from OSR was highest at pH 7.0, 36 h of incubation and in presence of 625 mu g/mL substrate concentration, 50 mu L/mL strain concentration respectively. Biocatalysis was considered the alternatives to preparation of sophoridine with green and sustainable synthetic processes except for less time-consuming and more environmentally friendly.
学科主题Chemistry, Multidisciplinary
出版地WEINHEIM
电子版国际标准刊号2192-6549
WOS关键词MICE ; EXPRESSION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000375554200003
出版者WILEY-V C H VERLAG GMBH
资助机构National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21462057] ; Program of Ministry of Education Chunhui Plan [Z2014091] ; Guizhou Science and Technology Department [QKHLH-2014-7555] ; Program for Guizhou Key Laboratory of Microbial Resources & Drug Development [GZMRD-2014-003] ; Ntional Training Program of Innovation and Entrepreneurship for Undergraduates [201410661013]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/24976]  
专题中科院植物分子生理学重点实验室
作者单位1.Zunyi Med Coll, Dept Pharm, Zunyi 563000, Peoples R China
2.CSIRO, Mat Sci & Engn, Sydney 2070, NSW, Australia
3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
4.Zunyi Med Coll, Guizhou Key Lab Microbial Resources & Drug Dev, Zunyi 563003, Peoples R China
5.Zunyi Med Coll, Dept Publ Hlth, Zunyi 563000, Peoples R China
推荐引用方式
GB/T 7714
Fu, Shaobin,Li, Yi,Meng, Qingfeng,et al. Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil[J]. JOURNAL OF THE CHINESE CHEMICAL SOCIETY,2016,63(4):331-335.
APA Fu, Shaobin,Li, Yi,Meng, Qingfeng,Yue, Changwu,Sun, Yongzhen,&Wei, Gang.(2016).Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil.JOURNAL OF THE CHINESE CHEMICAL SOCIETY,63(4),331-335.
MLA Fu, Shaobin,et al."Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil".JOURNAL OF THE CHINESE CHEMICAL SOCIETY 63.4(2016):331-335.

入库方式: OAI收割

来源:植物研究所

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