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Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis

文献类型：期刊论文

作者Shang, Baoshuan2; Xu, Chongyi; Zhang, Xixi2; Cao, Huifen2; Xin, Wei; Hu, Yuxin1
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2016
卷号113期号:18页码:5101-5106
关键词VLCFA pericycle callus formation regeneration
ISSN号0027-8424
DOI10.1073/pnas.1522466113
文献子类Article
英文摘要The already differentiated organs in plants have a remarkable capacity to regenerate new individuals under culture conditions. Plant in vitro regeneration practically starts with the induction of a pluripotent cell mass, the callus, from detached organs on auxin-rich callus-inducing medium (CIM), which is generally required for subsequent regeneration of new bodies. Recent studies show that CIM-induced callus formation occurs from the pericycle or pericycle-like cells through a root developmental pathway, whereas the signals involved in governing callus-forming capacity of pericycle cells remain unknown. Here we report that very-long-chain fatty acids (VLCFAs) play a critical role in confining the pericycle competence for callus formation and thus the regeneration capacity of Arabidopsis. By genetic screening, we identified the callus formation-related 1 (cfr1) mutant, which bypasses the inhibition of callus-forming capacity in roots by solitary-root (slr/iaa14). We show that CFR1 encodes 3-ketoacyl-CoA synthase 1 (KCS1), which catalyzes a rate-limiting step of VLCFA biosynthesis. Our biochemical and genetic analyses demonstrate that VLCFAs restrict the pericycle competence for callus formation, at least in part, by regulating the transcription of Aberrant Lateral Root Formation 4 (ALF4). Moreover, we provide evidence that VLCFAs act as cell layer signals to mediate the pericycle competence for callus formation. Taken together, our results identify VLCFAs or their derivatives as the confining signals for mediating the pericycle competence for callus formation and thus the regeneration capacity of plant organs.
学科主题Multidisciplinary Sciences
出版地WASHINGTON
WOS关键词LATERAL ROOT-FORMATION ; DEPENDENT AUXIN GRADIENTS ; STEM-CELL NICHE ; THALIANA ; BIOSYNTHESIS ; PLANTS ; GENES ; SPHINGOLIPIDS ; ACTIVATION ; CYTOKININ
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000375395700055
出版者NATL ACAD SCIENCES
资助机构Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013CB967300] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31230009, 31371447]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25333]  
专题中科院植物分子生理学重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Shang, Baoshuan,Xu, Chongyi,Zhang, Xixi,et al. Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016,113(18):5101-5106.
APA Shang, Baoshuan,Xu, Chongyi,Zhang, Xixi,Cao, Huifen,Xin, Wei,&Hu, Yuxin.(2016).Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,113(18),5101-5106.
MLA Shang, Baoshuan,et al."Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113.18(2016):5101-5106.

入库方式： OAI收割

来源：植物研究所

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