The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis
文献类型：期刊论文
| 作者 | Li, Qi2; Deng, Zhuyun; Gong, Chunyan; Wang, Tai
|
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 7 |
| 关键词 | pollen development microgametogenesis translation initiation eIF3f rice |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2016.00532 |
| 文献子类 | Article |
| 英文摘要 | Microgametogenesis is the post-meiotic pollen developmental phase when unicellular microspores develop into mature tricellular pollen. In rice, microgametogenesis can influence grain yields to a great degree because pollen abortion occurs more easily during microgametogenesis than during other stages of pollen development. However, our knowledge of the genes involved in microgametogenesis in rice remains limited. Due to the dependence of pollen development on the regulatory mechanisms of protein expression, we identified the encoding gene of the eukaryotic translation initiation factor 3, subunit fin Oryza saliva (OseIF3f). Immunoprecipitation combined with mass spectrometry confirmed that OseIF3f was a subunit of rice elF3, which consisted of at least 12 subunits including elF3a, eIF3b, elF3c, elF3d, elF3e, elF3f, elF3g, elF3h, elF3i, elF3k, elF3l, and elF3m. OseIF3f showed high mRNA levels in immature florets and is highly abundant in developing anthers. Subcellular localization analysis showed that OseIF3f was localized to the cytosol and the endoplasmic reticulum in rice root cells. We further analyzed the biological function of OseIF3f using the double-stranded RNA-mediated interference (RNAi) approach. The OseIF3f-RNAi lines grew normally at the vegetative stage but displayed a large reduction in seed production and pollen viability, which is associated with the down-regulation of OseIF3f. Further cytological observations of pollen development revealed that the OseIF3f-RNAi lines showed no obvious abnormalities at the male meiotic stage and the unicellular microspore stage. However, compared to the wild-type, OseIF3f-RNAi lines contained a higher percentage of arrested unicellular pollen at the bicellular stage and a higher percentage of arrested unicellular and bicellular pollen, and aborted pollen at the tricellular stage. These results indicate that OseiF3f plays a role in microgametogenesis. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | POLLEN DEVELOPMENT ; GENE-EXPRESSION ; PROTEIN ; MEIOSIS ; ENCODES ; EIF3 ; ORTHOLOGUE ; REGULATOR ; REVEALS ; COMPLEX |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000374731900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2011CB944602, 2012CB910504] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25355]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qi,Deng, Zhuyun,Gong, Chunyan,et al. The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis[J]. FRONTIERS IN PLANT SCIENCE,2016,7. |
| APA | Li, Qi,Deng, Zhuyun,Gong, Chunyan,&Wang, Tai.(2016).The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis.FRONTIERS IN PLANT SCIENCE,7. |
| MLA | Li, Qi,et al."The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis".FRONTIERS IN PLANT SCIENCE 7(2016). |
入库方式： OAI收割
来源：植物研究所
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