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Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses

文献类型：期刊论文

作者Zhao, Qi4; Suo, Jinwei4; Chen, Sixue5; Jin, Yudan4; Ma, Xiaolin4; Yin, Zepeng; Zhang, Yuhong; Wang, Tai1; Luo, Ji3; Jin, Wenhai3
刊名SCIENTIFIC REPORTS
出版日期2016
卷号6
ISSN号2045-2322
DOI10.1038/srep32717
文献子类Article
英文摘要Soil alkalization severely affects crop growth and agricultural productivity. Alkali salts impose ionic, osmotic, and high pH stresses on plants. The alkali tolerance molecular mechanism in roots from halophyte Puccinellia tenuiflora is still unclear. Here, the changes associated with Na2CO3 tolerance in P. tenuiflora roots were assessed using physiological and iTRAQ-based quantitative proteomic analyses. We set up the first protein dataset in P. tenuiflora roots containing 2,671 non-redundant proteins. Our results showed that Na2CO3 slightly inhibited root growth, caused ROS accumulation, cell membrane damage, and ion imbalance, as well as reduction of transport and protein synthesis/turnover. The Na2CO3-responsive patterns of 72 proteins highlighted specific signaling and metabolic pathways in roots. Ca2+ signaling was activated to transmit alkali stress signals as inferred by the accumulation of calcium-binding proteins. Additionally, the activities of peroxidase and glutathione peroxidase, and the peroxiredoxin abundance were increased for ROS scavenging. Furthermore, ion toxicity was relieved through Na+ influx restriction and compartmentalization, and osmotic homeostasis reestablishment due to glycine betaine accumulation. Importantly, two transcription factors were increased for regulating specific alkali-responsive gene expression. Carbohydrate metabolism-related enzymes were increased for providing energy and carbon skeletons for cellular metabolism. All these provide new insights into alkali-tolerant mechanisms in roots.
学科主题Multidisciplinary Sciences
出版地LONDON
WOS关键词STRESS-RESPONSIVE PROTEINS ; EXPRESSED SEQUENCE TAGS ; SALT-STRESS ; GENE-EXPRESSION ; ENVIRONMENTAL-STRESSES ; OVER-EXPRESSION ; SEEDLING ROOTS ; SALINE-ALKALI ; CONFERS SALT ; TOLERANCE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000382467100001
出版者NATURE PUBLISHING GROUP
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270310] ; Fundamental Research Funds for the Central Universities, ChinaFundamental Research Funds for the Central Universities [2572014EA04] ; Special Fund for Forestry-Scientific Research in the Public Interest [201104066]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25367]  
专题中科院植物分子生理学重点实验室
作者单位1.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Genet Inst, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA
2.AB Sciex Asia Pacific Applicat Support Ctr, Shanghai 200233, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
4.Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China
5.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Salinealkali Vegetat Ecol Restorat Oil Fi, Minist Educ, Harbin 150040, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Qi,Suo, Jinwei,Chen, Sixue,et al. Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses[J]. SCIENTIFIC REPORTS,2016,6.
APA Zhao, Qi.,Suo, Jinwei.,Chen, Sixue.,Jin, Yudan.,Ma, Xiaolin.,...&Dai, Shaojun.(2016).Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses.SCIENTIFIC REPORTS,6.
MLA Zhao, Qi,et al."Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses".SCIENTIFIC REPORTS 6(2016).

入库方式： OAI收割

来源：植物研究所

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