Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803
文献类型：期刊论文
| 作者 | Dong, Wei3; Wang, Jia3; Niu, Guoqi1; Zhao, Shun; Liu, Lin |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2016 |
| 卷号 | 590期号:19页码:3435-3442 |
| 关键词 | HtrA protease oligomerization PDZ domain redox regulation zinc binding |
| ISSN号 | 0014-5793 |
| DOI | 10.1002/1873-3468.12416 |
| 文献子类 | Article |
| 英文摘要 | The high temperature requirement A (HtrA) proteases are oligomeric serine proteases essential for protein quality control. HtrA homolog A (HhoA) from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 assembles into a proteolytically active hexamer. Herein, we present the crystal structure of the hexameric HhoA in complex with the copurified peptide. Our data indicate the presence of three methionines in close proximity to the peptide-binding site of the PDZ domain. Unexpectedly, we observed that a zinc ion is accommodated within the central channel formed by a HhoA trimer. However, neither calcium nor magnesium showed affinity for HhoA. The role of the zinc ion in HhoA was tested in an in vitro proteolytic assay against the nonspecific substrate -casein and was found to be inhibitory. Our findings provide insights into the regulation of HhoA by a redox-related mechanism involving methionine residues and by zinc ion-binding within the central channel. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1873-3468 |
| WOS关键词 | PHOTOSYSTEM-II ; DEG/HTRA PROTEASES ; QUALITY CONTROL ; HTRA PROTEASES ; DEG PROTEASES ; CELL ; FAMILY ; DISTINCT ; REPAIR ; ADAPTATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000386153900013 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170688] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25293]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Wei,Wang, Jia,Niu, Guoqi,et al. Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803[J]. FEBS LETTERS,2016,590(19):3435-3442. |
| APA | Dong, Wei,Wang, Jia,Niu, Guoqi,Zhao, Shun,&Liu, Lin.(2016).Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803.FEBS LETTERS,590(19),3435-3442. |
| MLA | Dong, Wei,et al."Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803".FEBS LETTERS 590.19(2016):3435-3442. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。