Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions
文献类型：期刊论文
| 作者 | Li, Lingyu1; Yang, Haomeng; Ren, Weibo4; Liu, Bei1; Cheng, Dongmei1; Wu, Xinhong4; Gong, Jirui3; Peng, Lianwei; Huang, Fang
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| 刊名 | JOURNAL OF PLANT BIOLOGY
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| 出版日期 | 2016 |
| 卷号 | 59期号:4页码:336-346 |
| 关键词 | Leymus chinensis Phosphate starvation Photosystem II ROS-scavenging enzymes Thylakoid membranes 2D-BN/PAGE |
| ISSN号 | 1226-9239 |
| DOI | 10.1007/s12374-016-0117-1 |
| 文献子类 | Article |
| 英文摘要 | Sheepgrass [Leymus chinensis (Trin.) Tzvel] is a valuble forage plant highly significant to regional grassland productivity of Euro-Asia steppes. Although effects of environmental stress including drought have been studied, impact of nutrient deficiency in particular phosphate (Pi), one of the essential macronutrient, is not reported. Here, we investigated low-Pi effect on its photosynthetic apparatus via physiological and biochemical analysis. We show that PSII activity was significantly reduced based on chlorophyll fluorescence measurements. We observed decreased amount of core proteins of PSII by immunoblot analysis. Further analysis of thylakoid membranes using 2D-BN/SDS-PAGE and immunoblot detection demonstrated that the amount of PSII complexes was closely correlated with the Pi levels within the range. Together with reduced number of thylakoid grana stackings observed, we suggest that the maintenance of PSII is impaired under Pi-limited condition. Moreover, enzyme activity assays revealed that the activity of several ROS scavenger enzymes was stimulated by low-Pi treatment. Based on these experimental results we conclude that PSII is the component of photosynthetic apparatus most sensitive to Pi supply and the enhanced anti-ROS activity is mainly subjective to protection of PSII against low Pi-induced photo-oxidative stress in the organism. |
| 学科主题 | Plant Sciences |
| 出版地 | HEIDELBERG |
| 电子版国际标准刊号 | 1867-0725 |
| WOS关键词 | PHOTOOXIDATIVE STRESS ; MOLECULAR-MECHANISMS ; PHOSPHORUS-NUTRITION ; LIPID-PEROXIDATION ; PHOTOSYSTEM-II ; GLYCINE-MAX ; ARABIDOPSIS ; PLANTS ; TOLERANCE ; ASSAY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000381137700004 |
| 出版者 | SPRINGER HEIDELBERG |
| 资助机构 | National Key Basic Research Development Program of ChinaNational Basic Research Program of China [2014CB138800] ; Key Research Program of the Chinese Academy of Science [KGZD-EW-T05] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25302]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Beijing Normal Univ, Coll Resources Sci & Technol, Beijing 100875, Peoples R China 3.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lingyu,Yang, Haomeng,Ren, Weibo,et al. Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions[J]. JOURNAL OF PLANT BIOLOGY,2016,59(4):336-346. |
| APA | Li, Lingyu.,Yang, Haomeng.,Ren, Weibo.,Liu, Bei.,Cheng, Dongmei.,...&Huang, Fang.(2016).Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions.JOURNAL OF PLANT BIOLOGY,59(4),336-346. |
| MLA | Li, Lingyu,et al."Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions".JOURNAL OF PLANT BIOLOGY 59.4(2016):336-346. |
入库方式： OAI收割
来源：植物研究所
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