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BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex

文献类型：期刊论文

作者Zhang, Lin2; Duan, Zhikun2; Zhang, Jiao; Peng, Lianwei
刊名PLANT PHYSIOLOGY
出版日期2016
卷号171期号:2页码:1291-1306
ISSN号0032-0889
DOI10.1104/pp.16.00248
文献子类Article
英文摘要Thylakoid membrane-localized chloroplast ATP synthases use the proton motive force generated by photosynthetic electron transport to produce ATP from ADP. Although it is well known that the chloroplast ATP synthase is composed of more than 20 proteins with alpha(3)beta(3)gamma(1)epsilon(1)delta 1I1II1III14IV1 stoichiometry, its biogenesis process is currently unclear. To unravel the molecular mechanisms underlying the biogenesis of chloroplast ATP synthase, we performed extensive screening for isolating ATP synthase mutants in Arabidopsis (Arabidopsis thaliana). In the recently identified bfa3 (biogenesis factors required for ATP synthase 3) mutant, the levels of chloroplast ATP synthase subunits were reduced to approximately 25% of wild-type levels. In vivo labeling analysis showed that assembly of the CF1 component of chloroplast ATP synthase was less efficient in bfa3 than in the wild type, indicating that BFA3 is required for CF1 assembly. BFA3 encodes a chloroplast stromal protein that is conserved in higher plants, green algae, and a few species of other eukaryotic algae, and specifically interacts with the CF1 beta subunit. The BFA3 binding site was mapped to a region in the catalytic site of CF1 beta. Several residues highly conserved in eukaryotic CF1 beta are crucial for the BFA3-CF1 beta interaction, suggesting a coevolutionary relationship between BFA3 and CF1 beta. BFA3 appears to function as a molecular chaperone that transiently associates with unassembled CF1 beta at its catalytic site and facilitates subsequent association with CF1 alpha during assembly of the CF1 subcomplex of chloroplast ATP synthase.
学科主题Plant Sciences
出版地ROCKVILLE
电子版国际标准刊号1532-2548
WOS关键词CYCLIC ELECTRON FLOW ; COUPLING FACTOR-I ; ARABIDOPSIS-THALIANA ; PHOTOSYNTHETIC COMPLEX ; GAMMA-SUBUNIT ; BINDING-SITE ; PROTEIN ; F-1-ATPASE ; PROMOTES ; PHOTOPROTECTION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000380699200040
出版者AMER SOC PLANT BIOLOGISTS
资助机构National Natural Science FundsNational Natural Science Foundation of China (NSFC) [31322007] ; Hundred Talents Program of the Chinese Academy of SciencesChinese Academy of Sciences
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25315]  
专题中科院光生物学重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Lin,Duan, Zhikun,Zhang, Jiao,et al. BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex[J]. PLANT PHYSIOLOGY,2016,171(2):1291-1306.
APA Zhang, Lin,Duan, Zhikun,Zhang, Jiao,&Peng, Lianwei.(2016).BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex.PLANT PHYSIOLOGY,171(2),1291-1306.
MLA Zhang, Lin,et al."BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex".PLANT PHYSIOLOGY 171.2(2016):1291-1306.

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来源：植物研究所

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