Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Li, Hongmei; Pu, Hua1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2016 |
| 卷号 | 474期号:1页码:226-231 |
| 关键词 | Arabidopsis thaliana Chlorophyll breakdown MES16 Demethylation alpha/beta-hydrolase superfamily Catalytic triad |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2016.04.115 |
| 文献子类 | Article |
| 英文摘要 | Methylesterase family member 16 (MES16) is an integral component of chlorophyll breakdown. It catalyzes the demethylation of fluorescent chlorophyll catabolite (FCC) and pheophorbide in vitro, and specifically demethylates FCC in vivo. Here we report the crystal structure of MES16 from Arabidopsis thaliana at 2.8 angstrom resolution. The structure confirm that MES16 is a member of the alpha/beta-hydrolase superfamily with Ser-87, His-239, and Asp-211 as the catalytic triad. Our biochemical studies reveal that MES16 has esterase activity with methyl-indole acetic acid as the substrate, and the catalytically essential role of Ser-87 has been demonstrated. (C) 2016 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 出版地 | SAN DIEGO |
| 电子版国际标准刊号 | 1090-2104 |
| WOS关键词 | CHLOROPHYLL BREAKDOWN ; HYDROXYNITRILE LYASE ; CATABOLITES ; DEGRADATION ; ENZYME ; BIOSYNTHESIS ; ESTERASE ; CLONING ; LEAVES ; PATH |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000376810600034 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300634] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25316]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 101048, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hongmei,Pu, Hua. Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,474(1):226-231. |
| APA | Li, Hongmei,&Pu, Hua.(2016).Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,474(1),226-231. |
| MLA | Li, Hongmei,et al."Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 474.1(2016):226-231. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。