Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening
文献类型：期刊论文
| 作者 | Ning, De-Li; Liu, Ke-Hui; Liu, Chang-Cai; Liu, Jin-Wen; Qian, Chun-Rong; Yu, Yang7; Wang, Yue-Feng; Wang, Ying-Chun; Wang, Bai-Chen |
| 刊名 | PLANTA
 |
| 出版日期 | 2016 |
| 卷号 | 243期号:2页码:501-517 |
| 关键词 | De-etiolation Maize Phosphoprotein Proteomics Signal transduction |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-015-2420-3 |
| 文献子类 | Article |
| 英文摘要 | Main conclusion Large-scale comparative phosphoprotein analysis in maize seedlings reveals a complicated molecular regulation mechanism at the phosphoproteomic level during de-etiolation. In the present study we report a phosphoproteomic study conducted on Zea mays etiolated leaves harvested at three time points during greening (etiolated seedlings and seedlings exposed to light for 6 or 12 h). We identified a total of 2483 phosphopeptides containing 2389 unambiguous phosphosites from 1339 proteins. The abundance of nearly 692 phosphorylated peptides containing 783 phosphosites was reproducible and profiled with high confidence among treatments. Comparisons with other large-scale phosphoproteomic studies revealed that 473 of the phosphosites are novel to this study. Of the 783 phosphosites identified, 171, 79, and 138 were identified in 0, 6, and 12 h samples, respectively, which suggest that regulation of phosphorylation plays important roles during maize seedling de-etiolation. Our experimental methods included enrichment of phosphoproteins, allowing the identification of a great number of low abundance proteins, such as transcription factors, protein kinases, and photoreceptors. Most of the identified phosphoproteins were involved in gene transcription, post-transcriptional regulation, or signal transduction, and only a few were involved in photosynthesis and carbon metabolism. It is noteworthy that tyrosine phosphorylation and calcium signaling pathways might play important roles during maize seedling de-etiolation. Taken together, we have elucidated a new level of complexity in light-induced reversible protein phosphorylation during maize seedling de-etiolation. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; NUCLEIC-ACID-BINDING ; PHOSPHOENOLPYRUVATE CARBOXYKINASE ; SIGNAL-TRANSDUCTION ; PHOSPHORYLATION ; ARABIDOPSIS ; LIGHT ; IDENTIFICATION ; GROWTH ; PHYTOCHROME |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000373310300017 |
| 出版者 | SPRINGER |
| 资助机构 | State Key Program of the National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31030017] ; Natural Sciences Foundation of Heilongjiang ProvinceNatural Science Foundation of Heilongjiang Province [C201010] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25325]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 2.[Liu, Ke-Hui 3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 4.Res Inst Chem Def, State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China 5.Heilongjiang Bayi Agr Univ, Coll Life Sci & Biotechnol, Daqing 163319, Peoples R China 6.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China 7.[Qian, Chun-Rong 8.Qiqihar Med Univ, Inst Med, Qiqihar 161006, Peoples R China 9.[Ning, De-Li 10.Wang, Yue-Feng |
| 推荐引用方式 GB/T 7714 | Ning, De-Li,Liu, Ke-Hui,Liu, Chang-Cai,et al. Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening[J]. PLANTA,2016,243(2):501-517. |
| APA | Ning, De-Li.,Liu, Ke-Hui.,Liu, Chang-Cai.,Liu, Jin-Wen.,Qian, Chun-Rong.,...&Wang, Bai-Chen.(2016).Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening.PLANTA,243(2),501-517. |
| MLA | Ning, De-Li,et al."Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening".PLANTA 243.2(2016):501-517. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。