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The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein

文献类型：期刊论文

作者Fang, Ying4; Zhao, Shun4; Zhang, Feilong1; Zhao, Aiguo3; Zhang, Wenxia; Zhang, Min1; Liu, Lin
刊名SCIENTIFIC REPORTS
出版日期2016
卷号6
ISSN号2045-2322
DOI10.1038/srep19756
文献子类Article
英文摘要Tetrapyrrole biosynthesis is an essential and tightly regulated process, and glutamyl-tRNA reductase (GluTR) is a key target for multiple regulatory factors at the post-translational level. By binding to the thylakoid membrane protein FLUORESCENT (FLU) or the soluble stromal GluTR-binding protein (GBP), the activity of GluTR is down-or up-regulated. Here, we reconstructed a ternary complex composed of the C-terminal tetratricopepetide-repeat domain of FLU, GBP, and GluTR, crystallized and solved the structure of the complex at 3.2 angstrom. The overall structure resembles the shape of merged two binary complexes as previously reported, and shows a large conformational change within GluTR. We also demonstrated that GluTR binds tightly with GBP but does not bind to GSAM under the same condition. These findings allow us to suggest a biological role of the ternary complex for the regulation of plant GluTR.
学科主题Multidisciplinary Sciences
出版地LONDON
WOS关键词5-AMINOLEVULINIC ACID SYNTHESIS ; TETRAPYRROLE BIOSYNTHESIS ; CHLOROPHYLL BIOSYNTHESIS ; NEGATIVE REGULATOR ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; ENZYME ; EXPRESSION ; THALIANA ; HEMA1
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000368667700002
出版者NATURE PUBLISHING GROUP
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370759, 31500243] ; National Basic Research Program of ChinaNational Basic Research Program of China [2011CBA00901] ; CASChinese Academy of Sciences [KGZD-EW-T05]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25326]  
专题中科院光生物学重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Northwest A&F Univ, Coll Life Sci, Xian 712100, Shaanxi, Peoples R China
3.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
4.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Fang, Ying,Zhao, Shun,Zhang, Feilong,et al. The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein[J]. SCIENTIFIC REPORTS,2016,6.
APA Fang, Ying.,Zhao, Shun.,Zhang, Feilong.,Zhao, Aiguo.,Zhang, Wenxia.,...&Liu, Lin.(2016).The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein.SCIENTIFIC REPORTS,6.
MLA Fang, Ying,et al."The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein".SCIENTIFIC REPORTS 6(2016).

入库方式： OAI收割

来源：植物研究所

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