Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis
文献类型：期刊论文
| 作者 | Ding, Shunhua; Wang, Liang2; Yang, Zhipan; Lu, Qingtao ; Wen, Xiaogang; Lu, Congming
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| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 58期号:1页码:29-47 |
| 关键词 | Arabidopsis thaliana ascorbate-glutathione cycle glutathione reductase2 hydrogen peroxide leaf senescence |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.12371 |
| 文献子类 | Article |
| 英文摘要 | Glutathione reductase (GR) catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH) and participates in the ascorbate-glutathione cycle, which scavenges H2O2. Here, we report that chloroplastic/mitochondrial GR2 is an important regulator of leaf senescence. Seed development of the homozygous gr2 knockout mutant was blocked at the globular stage. Therefore, to investigate the function of GR2 in leaf senescence, we generated transgenic Arabidopsis plants with decreased GR2 using RNAi. The GR2 RNAi plants displayed early onset of age-dependent and dark- and H2O2-induced leaf senescence, which was accompanied by the induction of the senescence-related marker genes SAG12 and SAG13. Furthermore, transcriptome analysis revealed that genes related to leaf senescence, oxidative stress, and phytohormone pathways were upregulated directly before senescence in RNAi plants. In addition, H2O2 accumulated to higher levels in RNAi plants than in wild-type plants and the levels of H2O2 peaked in RNAi plants directly before the early onset of leaf senescence. RNAi plants showed a greater decrease in GSH/GSSG levels than wild-type plants during leaf development. Our results suggest that GR2 plays an important role in leaf senescence by modulating H2O2 and glutathione signaling in Arabidopsis. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | OXIDATIVE STRESS RESPONSES ; GENE-EXPRESSION ; HYDROGEN-PEROXIDE ; TRANSCRIPTION FACTOR ; SALICYLIC-ACID ; TRANSGENIC TOBACCO ; CELL-DEATH ; CHLOROPLASTS ; H2O2 ; BIOSYNTHESIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000367731700003 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30970218] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25328]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Shunhua,Wang, Liang,Yang, Zhipan,et al. Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2016,58(1):29-47. |
| APA | Ding, Shunhua,Wang, Liang,Yang, Zhipan,Lu, Qingtao,Wen, Xiaogang,&Lu, Congming.(2016).Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,58(1),29-47. |
| MLA | Ding, Shunhua,et al."Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 58.1(2016):29-47. |
入库方式： OAI收割
来源：植物研究所
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