Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids
文献类型：期刊论文
| 作者 | Liu, Cheng; Gao, Zhimin2; Liu, Kun; Sun, Ruixue; Cui, Chunbo; Holzwarth, Alfred R.; Yang, Chunhong |
| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 127期号:1页码:109-116 |
| 关键词 | Photosynthesis PsbS Proteoliposomes Reconstitution Refolding |
| ISSN号 | 0166-8595 |
| DOI | 10.1007/s11120-015-0176-z |
| 文献子类 | Article |
| 英文摘要 | The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study, we simultaneously refolded and reconstituted functional pea PsbS into liposomes of thylakoid lipids starting from denatured expressed protein. Intrinsic fluorescence spectroscopy, trypsin digestion, and circular dichroism spectroscopy were used to characterize the native state of PsbS in the proteoliposomes. The functionality of refolded PsbS was further demonstrated by its effect on the fluorescence quenching of the major antenna system of photosystem II (LHCII) co-inserted into the liposomes. The fluorescence yield of native trimeric LHCII was lowered by PsbS by 50 % at neutral pH and by a further 25 % upon lowering the pH to 4.5. Furthermore, the acid-induced fluorescence reduction was completely reversed by addition of N,N'-dicyclohexylcarbodiimide, an inhibitor of protein protonation. These results indicate that reconstituted PsbS induces strong quenching of LHCII sensing changes in local pH via its protonation sites. |
| 学科主题 | Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5079 |
| WOS关键词 | LIGHT-HARVESTING COMPLEX ; TIME-RESOLVED FLUORESCENCE ; PHOTOSYSTEM-II COMPLEXES ; PROTEIN CONTROLS ; IN-VITRO ; MEMBRANES ; SUBUNIT ; ANTENNA ; IDENTIFICATION ; ORGANIZATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000366156400013 |
| 出版者 | SPRINGER |
| 资助机构 | National Basic Research Program of ChinaNational Basic Research Program of China [2011CBA00904] ; Key Research Program of the Chinese Academy of Sciences Grant [KSZD-EW-Z-018] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30800069, 31070212, 31370275, 31370588] ; European Union EU Training and Research Network Harvest ; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG HO-924/3-1] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25329]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.State Forestry Adm, Int Ctr Bamboo & Rattan, Key Open Lab Bamboo & Rattan Sci & Technol, Beijing 100102, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Holzwarth, Alfred R.] Max Planck Inst Chem Energiekonvers, D-45470 Mulheim, Germany |
| 推荐引用方式 GB/T 7714 | Liu, Cheng,Gao, Zhimin,Liu, Kun,et al. Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids[J]. PHOTOSYNTHESIS RESEARCH,2016,127(1):109-116. |
| APA | Liu, Cheng.,Gao, Zhimin.,Liu, Kun.,Sun, Ruixue.,Cui, Chunbo.,...&Yang, Chunhong.(2016).Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids.PHOTOSYNTHESIS RESEARCH,127(1),109-116. |
| MLA | Liu, Cheng,et al."Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids".PHOTOSYNTHESIS RESEARCH 127.1(2016):109-116. |
入库方式： OAI收割
来源：植物研究所
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