Proteome analysis of alfalfa roots in response to water deficit stress
文献类型：期刊论文
| 作者 | Atikur, Rahman Md; Yong-goo, Kim; Iftekhar, Alam3; Liu Gong-she ; Hyoshin, Lee1; Joo, Lee Jeung5; Byung-Hyun, Lee
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| 刊名 | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| 出版日期 | 2016 |
| 卷号 | 15期号:6页码:1275-1285 |
| 关键词 | alfalfa proteome root water deficit stress |
| ISSN号 | 2095-3119 |
| DOI | 10.1016/S2095-3119(15)61255-2 |
| 文献子类 | Article |
| 英文摘要 | To evaluate the response of alfalfa to water deficit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H2O2 content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots. |
| 学科主题 | Agriculture, Multidisciplinary |
| 出版地 | OXFORD |
| WOS关键词 | TERM DROUGHT ; SYNTHETASE ; PROTEINS ; OVEREXPRESSION ; TOLERANCE ; PLANT |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000378188600010 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Research Foundation of Korea (NRF)National Research Foundation of Korea [NRF-2011-616-F00013] ; BK21Plus program, Ministry of Education, Republic of Korea |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/24982]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Natl Inst Biotechnol, Dhaka 1349, Bangladesh 2.Gyeongsang Natl Univ, IALS, Div Appl Life Sci BK21Plus, Jinju 660701, South Korea 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Gyeongsang Natl Univ, IALS, Dept Appl Biol, Jinju 660701, South Korea 5.Korea Forest Res Inst, Div Forest Biotechnol, Suwon 441847, South Korea |
| 推荐引用方式 GB/T 7714 | Atikur, Rahman Md,Yong-goo, Kim,Iftekhar, Alam,et al. Proteome analysis of alfalfa roots in response to water deficit stress[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(6):1275-1285. |
| APA | Atikur, Rahman Md.,Yong-goo, Kim.,Iftekhar, Alam.,Liu Gong-she.,Hyoshin, Lee.,...&Byung-Hyun, Lee.(2016).Proteome analysis of alfalfa roots in response to water deficit stress.JOURNAL OF INTEGRATIVE AGRICULTURE,15(6),1275-1285. |
| MLA | Atikur, Rahman Md,et al."Proteome analysis of alfalfa roots in response to water deficit stress".JOURNAL OF INTEGRATIVE AGRICULTURE 15.6(2016):1275-1285. |
入库方式： OAI收割
来源：植物研究所
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