Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds
文献类型：期刊论文
| 作者 | Liu, Shu-Jun; Xu, Heng-Heng; Wang, Wei-Qing; Li, Ni; Wang, Wei-Ping; Lu, Zhuang; Moller, Ian Max3; Song, Song-Quan |
| 刊名 | JOURNAL OF PLANT PHYSIOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 196-197页码:79-92 |
| 关键词 | Embryo Oryza sativa Proteome analysis Seed germination Seedling establishment |
| ISSN号 | 0176-1617 |
| DOI | 10.1016/j.jplph.2016.02.021 |
| 文献子类 | Article |
| 英文摘要 | Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 mu M inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 mu M completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development. (C) 2016 Elsevier GmbH. All rights reserved. |
| 学科主题 | Plant Sciences |
| 出版地 | MUNICH |
| 电子版国际标准刊号 | 1618-1328 |
| WOS关键词 | PROTEOMIC ANALYSIS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; MESSENGER-RNAS ; LIPOXYGENASE ; MOBILIZATION ; GROWTH ; ROLES |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000376162400009 |
| 出版者 | ELSEVIER GMBH |
| 资助机构 | National Science and Technology Support Program [2012BAC01B05] ; Chinese Academy of Sciences Visiting Professorship for senior international scientists |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25246]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Hunan Hybrid Rice Res Center, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China 4.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark |
| 推荐引用方式 GB/T 7714 | Liu, Shu-Jun,Xu, Heng-Heng,Wang, Wei-Qing,et al. Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds[J]. JOURNAL OF PLANT PHYSIOLOGY,2016,196-197:79-92. |
| APA | Liu, Shu-Jun.,Xu, Heng-Heng.,Wang, Wei-Qing.,Li, Ni.,Wang, Wei-Ping.,...&Song, Song-Quan.(2016).Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds.JOURNAL OF PLANT PHYSIOLOGY,196-197,79-92. |
| MLA | Liu, Shu-Jun,et al."Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds".JOURNAL OF PLANT PHYSIOLOGY 196-197(2016):79-92. |
入库方式： OAI收割
来源：植物研究所
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