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Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds

文献类型：期刊论文

作者Cao, Dechang2; Xu, Huimin; Zhao, Yuanyuan; Deng, Xin1; Liu, Yongxiu1; Soppe, Wim J. J.; Lin, Jinxing1
刊名PLANT PHYSIOLOGY
出版日期2016
卷号172期号:4页码:2347-2362
ISSN号0032-0889
DOI10.1104/pp.16.00384
文献子类Article
英文摘要Seeds with physiological dormancy usually experience primary and secondary dormancy in the nature; however, little is known about the differential regulation of primary and secondary dormancy. We combined multiple approaches to investigate cytological changes, hormonal levels, and gene expression dynamics in Cunninghamia lanceolata seeds during primary dormancy release and secondary dormancy induction. Light microscopy and transmission electron microscopy revealed that protein bodies in the embryo cells coalesced during primary dormancy release and then separated during secondary dormancy induction. Transcriptomic profiling demonstrated that expression of genes negatively regulating gibberellic acid (GA) sensitivity reduced specifically during primary dormancy release, whereas the expression of genes positively regulating abscisic acid (ABA) biosynthesis increased during secondary dormancy induction. Parallel analysis of RNA ends revealed uncapped transcripts for; 55% of all unigenes. A negative correlation between fold changes in expression levels of uncapped versus capped mRNAs was observed during primary dormancy release. However, this correlation was loose during secondary dormancy induction. Our analyses suggest that the reversible changes in cytology and gene expression during dormancy release and induction are related to ABA/GA balance. Moreover, mRNA degradation functions as a critical posttranscriptional regulator during primary dormancy release. These findings provide a mechanistic framework for understanding physiological dormancy in seeds.
学科主题Plant Sciences
出版地CARY
电子版国际标准刊号1532-2548
WOS关键词UNCAPPED MESSENGER-RNAS ; GENE-EXPRESSION ; ABSCISIC-ACID ; PARALLEL ANALYSIS ; ARABIDOPSIS CVI ; MIRNA TARGETS ; CHINESE FIR ; IDENTIFICATION ; GERMINATION ; BIOSYNTHESIS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000391173400022
出版者OXFORD UNIV PRESS INC
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370212, 31270224] ; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design ; Programme of Introducing Talents of Discipline to Universities (111 Project)Ministry of Education, China - 111 Project [B13007] ; Programme for Changjiang Scholars and Innovative Research Team in UniversityProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13047] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2013M530536, 2015M570943]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25248]  
专题中科院北方资源植物重点实验室
作者单位1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany
4.Soppe, Wim J. J.] Max Planck Inst Plant Breeding Res, Dept Plant Breeding & Genet, D-50829 Cologne, Germany
推荐引用方式
GB/T 7714		 Cao, Dechang,Xu, Huimin,Zhao, Yuanyuan,et al. Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds[J]. PLANT PHYSIOLOGY,2016,172(4):2347-2362.
APA Cao, Dechang.,Xu, Huimin.,Zhao, Yuanyuan.,Deng, Xin.,Liu, Yongxiu.,...&Lin, Jinxing.(2016).Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds.PLANT PHYSIOLOGY,172(4),2347-2362.
MLA Cao, Dechang,et al."Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds".PLANT PHYSIOLOGY 172.4(2016):2347-2362.

入库方式： OAI收割

来源：植物研究所

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