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A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening

文献类型：期刊论文

作者Qin, Guozheng; Zhu, Zhu; Wang, Weihao1; Cai, Jianghua1; Chen, Yong1; Li, Li3; Tian, Shiping1
刊名PLANT PHYSIOLOGY
出版日期2016
卷号172期号:3页码:1596-1611
ISSN号0032-0889
DOI10.1104/pp.16.01269
文献子类Article
英文摘要Fruit ripening is a complex process that involves a series of physiological and biochemical changes that ultimately influence fruit quality traits, such as color and flavor. Sugar metabolism is an important factor in ripening, and there is evidence that it influences various aspects of ripening, although the associated mechanism is not well understood. In this study, we identified and analyzed the expression of 36 genes involved in Suc metabolism in ripening tomato (Solanum lycopersicum) fruit. Chromatin immunoprecipitation and gel mobility shift assays indicated that SlVIF, which encodes a vacuolar invertase inhibitor, and SlVI, encoding a vacuolar invertase, are directly regulated by the global fruit ripening regulator RIPENING INHIBITOR (RIN). Moreover, we showed that SlVIF physically interacts with SlVI to control Suc metabolism. Repression of SlVIF by RNA interference delayed tomato fruit ripening, while overexpression of SlVIF accelerated ripening, with concomitant changes in lycopene production and ethylene biosynthesis. An isobaric tags for relative and absolute quantification-based quantitative proteomic analysis further indicated that the abundance of a set of proteins involved in fruit ripening was altered by suppressing SlVIF expression, including proteins associated with lycopene generation and ethylene synthesis. These findings provide evidence for the role of Suc in promoting fruit ripening and establish that SlVIF contributes to fruit quality and the RIN-mediated ripening regulatory mechanisms, which are of significant agricultural value.
学科主题Plant Sciences
出版地ROCKVILLE
电子版国际标准刊号1532-2548
WOS关键词PLANT DEVELOPMENT ; EXPRESSION ANALYSIS ; SUGAR COMPOSITION ; GENE-EXPRESSION ; MESSENGER-RNA ; POTATO-TUBERS ; ACID ; CLONING ; PROTEIN ; FRUCTOKINASE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000391172300019
出版者AMER SOC PLANT BIOLOGISTS
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2011CB100604] ; Youth Innovation Promotion Association CAS
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25253]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.USDA, Robert W Holley Ctr Agr Hlth, Agr Res Serv, Ithaca, NY 14853 USA
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Qin, Guozheng,Zhu, Zhu,Wang, Weihao,et al. A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening[J]. PLANT PHYSIOLOGY,2016,172(3):1596-1611.
APA Qin, Guozheng.,Zhu, Zhu.,Wang, Weihao.,Cai, Jianghua.,Chen, Yong.,...&Tian, Shiping.(2016).A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening.PLANT PHYSIOLOGY,172(3),1596-1611.
MLA Qin, Guozheng,et al."A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening".PLANT PHYSIOLOGY 172.3(2016):1596-1611.

入库方式： OAI收割

来源：植物研究所

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