Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy
文献类型：期刊论文
| 作者 | Li, Xiaojuan; Xing, Jingjing4,5; Qiu, Zongbo1; He, Qihua2; Lin, Jinxing |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2016 |
| 卷号 | 9期号:9页码:1229-1239 |
| 关键词 | fluorescence correlation spectroscopy microdomain membrane protein dynamics interaction |
| ISSN号 | 1674-2052 |
| DOI | 10.1016/j.molp.2016.06.017 |
| 文献子类 | Review |
| 英文摘要 | Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluorescence correlation spectroscopy (FCS) is a powerful technique to describe events that occur at the single-molecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dynamics and interactions of membrane proteins. We also discuss the potential applications of this technique in plant biology. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1752-9867 |
| WOS关键词 | CROSS-CORRELATION SPECTROSCOPY ; PLASMA-MEMBRANE ; LIPID RAFTS ; ANOMALOUS DIFFUSION ; LIVING CELLS ; REVEALS ; MICRODOMAINS ; RECEPTOR ; CLATHRIN ; FCS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000386585500001 |
| 出版者 | CELL PRESS |
| 资助机构 | State Key Program of National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31530084] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370212, 31270224] ; Program of Introducing Talents of Discipline to UniversitiesMinistry of Education, China - 111 Project [13007] ; Program for Changjiang Scholars and Innovative Research Team in UniversityProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13047] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25257]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China 4.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaojuan,Xing, Jingjing,Qiu, Zongbo,et al. Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy[J]. MOLECULAR PLANT,2016,9(9):1229-1239. |
| APA | Li, Xiaojuan,Xing, Jingjing,Qiu, Zongbo,He, Qihua,&Lin, Jinxing.(2016).Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy.MOLECULAR PLANT,9(9),1229-1239. |
| MLA | Li, Xiaojuan,et al."Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy".MOLECULAR PLANT 9.9(2016):1229-1239. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。