Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis
文献类型：期刊论文
| 作者 | Li, Jingrui1; Wei, Xuezhi1; Yu, Pengli; Deng, Xin2,3 ; Xu, Wenxiu; Ma, Mi; Zhang, Haiyan
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| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 57期号:8页码:1720-1731 |
| 关键词 | Arabidopsis Cadmium (Cd) CadR Chloroplast targeting Mn Fe |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcw093 |
| 文献子类 | Article |
| 英文摘要 | Cadmium (Cd) is a transition metal that is highly toxic in biological systems. Anthropogenic emissions of Cd have increased biogeochemical cycling and the amount of Cd in the biosphere. Here we studied the utility of a bacterial Cd-binding protein, CadR, for the remediation of Cd contamination. CadR was successfully targeted to chloroplasts using a constitutive Cauliflower mosaic virus (CaMV) 35S promoter or a shoot-specific Chl a/b-binding protein 2 gene (CAB2) promoter and an RbcS (small subunit of the Rubisco complex) transit peptide. Under short-term (2 d) exposure to Cd, the cadR transgenic plants showed up to a 2.9-fold Cd accumulation in roots compared with untransformed plants. Under medium term (7 d) exposure to Cd, the concentrations of Cd in leaves began to increase but there were no differences between the wild type and the cadR transgenic plants. Under long-term (16 d) exposure to Cd, the cadR transgenic plants accumulated greater amounts of Cd in leaves than the untransformed plants. Total Cd accumulation (mg per plant) in shoots and roots of the plants expressing cadR were significantly higher (up to 3.5-fold in shoots and 5.2-fold in roots) than those of the untransformed plants. We also found that targeting CadR to chloroplasts facilitated chloroplastic metal homeostasis and Chl b accumulation. Our results demonstrate that manipulating chelating capacity in chloroplasts or in the cytoplasm may be effective in modifying both the accumulation of and resistance to Cd. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1471-9053 |
| WOS关键词 | METAL HYPERACCUMULATION ; PSEUDOMONAS-AERUGINOSA ; CADMIUM RESISTANCE ; OXIDATIVE STRESS ; TRANSIT PEPTIDE ; SMALL-SUBUNIT ; IRON ; TRANSPORTER ; ROOT ; CHLOROPLASTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000384717100010 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270313, 31470292] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25260]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Shanxi Normal Univ, Coll Life Sci, Linfen 041004, Shanxi, Peoples R China 3.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 4.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Li, Jingrui,Wei, Xuezhi,Yu, Pengli,et al. Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis[J]. PLANT AND CELL PHYSIOLOGY,2016,57(8):1720-1731. |
| APA | Li, Jingrui.,Wei, Xuezhi.,Yu, Pengli.,Deng, Xin.,Xu, Wenxiu.,...&Zhang, Haiyan.(2016).Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis.PLANT AND CELL PHYSIOLOGY,57(8),1720-1731. |
| MLA | Li, Jingrui,et al."Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis".PLANT AND CELL PHYSIOLOGY 57.8(2016):1720-1731. |
入库方式： OAI收割
来源：植物研究所
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