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Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy

文献类型：期刊论文

作者Li, Boqiang; Peng, Huaimin2; Tian, Shiping
刊名FRONTIERS IN MICROBIOLOGY
出版日期2016
卷号7
关键词attachment biological control EMS mutagenization graymold yeast
ISSN号1664-302X
DOI10.3389/fmicb.2016.00601
文献子类Article
英文摘要Rhodotorula glutinis as an antagonism show good biocontrol performance against various post-harvest diseases in fruits. In the present study, strong attachment capability of R. glutinis to spores and hyphae of Botrytis cinerea was observed. Further analysis showed that certain protein components on the yeast cell surface played critical role during the interaction between R. glutinis and B. cinerea. The components mainly distributed at the poles of yeast cells and might contain glycosylation modification, as tunicamycin treated yeast cells lost attachment capability to B. cinerea. To investigate contributions of attachment capability of R. glutinis to its biocontrol efficacy, yeast cells were mutagenized with 3% methane-sulfonic acid ethyl ester (EMS), and a mutant CE4 with stable non-attaching phenotype was obtained. No significant difference was found on colony, cell morphology, reproductive ability, and capsule formation between the mutant and wild-type. However, there was a distinct difference in India ink positive staining patterns between the two strains. Moreover, wild-type strain of R. glutinis showed better performance on inhibiting spore germination and mycelia' growth of B. cinerea than CE4 strain when yeast cells and B. cinerea were co-cultured in vitro. In biocontrol assay, both wild-type and CE4 strains showed significant biocontrol efficacy against gray mold caused by B. cinerea in apple fruit, whereas, control effect of CE4 strain was lower than that of wild-type. Our findings provided new evidences that attachment capability of R. glutinis to B. cinerea contributed to its biocontrol efficacy.
学科主题Microbiology
出版地LAUSANNE
WOS关键词GREY MOLD DISEASE ; POSTHARVEST DISEASES ; BIOLOGICAL-CONTROL ; RHODOSPORIDIUM-TORULOIDES ; PENICILLIUM-EXPANSUM ; CRYPTOCOCCUS-LAURENTII ; PICHIA-GUILLIERMONDII ; POSSIBLE MODE ; APPLE FRUIT ; IN-VITRO
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000375394900001
出版者FRONTIERS MEDIA SA
资助机构Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015BAD16B01-3] ; Key Research Program of Chinese Academy of SciencesChinese Academy of Sciences [KITZD-SW-1.02] ; Youth Innovation Promotion Association CAS [2015063]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25264]  
专题中科院北方资源植物重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Li, Boqiang,Peng, Huaimin,Tian, Shiping. Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA Li, Boqiang,Peng, Huaimin,&Tian, Shiping.(2016).Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy.FRONTIERS IN MICROBIOLOGY,7.
MLA Li, Boqiang,et al."Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy".FRONTIERS IN MICROBIOLOGY 7(2016).

入库方式： OAI收割

来源：植物研究所

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